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Gender-based differences in cardiac remodeling and ILK expression after myocardial infarction.

Sofia RR, Serra AJ, Silva JA, Antonio EL, Manchini MT, Oliveira FA, Teixeira VP, Tucci PJ - Arq. Bras. Cardiol. (2014)

Bottom Line: MI size was similar between genders.MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders.Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-graduação em Ciências da Reabilitação, Universidade Nove de Julho, São Paulo, SP, Brazil.

ABSTRACT

Background: Gender can influence post-infarction cardiac remodeling.

Objective: To evaluate whether gender influences left ventricular (LV) remodeling and integrin-linked kinase (ILK) after myocardial infarction (MI).

Methods: Female and male Wistar rats were assigned to one of three groups: sham, moderate MI (size: 20-39% of LV area), and large MI (size: ≥40% of LV area). MI was induced by coronary occlusion, and echocardiographic analysis was performed after six weeks to evaluate MI size as well as LV morphology and function. Real-time RT-PCR and Western blot were used to quantify ILK in the myocardium.

Results: MI size was similar between genders. MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders. Female rats with large MI showed a lower diastolic and systolic dilatation than the respective male rats; however, LV dysfunction was similar between genders. Gene and protein levels of ILK were increased in female rats with moderate and large infarctions, but only male rats with large infarctions showed an altered ILK mRNA level. A negative linear correlation was evident between LV dimensions and ILK expression in female rats with large MI.

Conclusions: Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

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Related in: MedlinePlus

Correlation analysis between ILK gene (panels: A-B) and protein (panels: C-D)expression and LV diastolic (LVDA) and systolic (LVSA) areas indexed to bodyweight in female rats with large MI size.
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f02: Correlation analysis between ILK gene (panels: A-B) and protein (panels: C-D)expression and LV diastolic (LVDA) and systolic (LVSA) areas indexed to bodyweight in female rats with large MI size.

Mentions: As previously described, female rats with large infarcts showed a lower LVdilatation. In addition, following MI there was increased ILK expression in femalerats compared with male rats. Since previous studies with gene therapy approacheshave shown that increased myocardial expression of ILK may reduce LV dilationfollowing MI12, correlationanalysis between LV areas and ILK levels were performed in female rats with large MIsize (Figure 2). We determined that a negativelinear correlation existed between LVDA, LVSA and ILK expression. The associationbetween LV areas and ILK remained statistically significant even after theapplication of a linear multivariable regression model (Table 3). Thus, an increased ILK expression was associated withsmaller LVDA as well as LVSA in female rats. However, a similar result was notobserved in male rats.


Gender-based differences in cardiac remodeling and ILK expression after myocardial infarction.

Sofia RR, Serra AJ, Silva JA, Antonio EL, Manchini MT, Oliveira FA, Teixeira VP, Tucci PJ - Arq. Bras. Cardiol. (2014)

Correlation analysis between ILK gene (panels: A-B) and protein (panels: C-D)expression and LV diastolic (LVDA) and systolic (LVSA) areas indexed to bodyweight in female rats with large MI size.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150663&req=5

f02: Correlation analysis between ILK gene (panels: A-B) and protein (panels: C-D)expression and LV diastolic (LVDA) and systolic (LVSA) areas indexed to bodyweight in female rats with large MI size.
Mentions: As previously described, female rats with large infarcts showed a lower LVdilatation. In addition, following MI there was increased ILK expression in femalerats compared with male rats. Since previous studies with gene therapy approacheshave shown that increased myocardial expression of ILK may reduce LV dilationfollowing MI12, correlationanalysis between LV areas and ILK levels were performed in female rats with large MIsize (Figure 2). We determined that a negativelinear correlation existed between LVDA, LVSA and ILK expression. The associationbetween LV areas and ILK remained statistically significant even after theapplication of a linear multivariable regression model (Table 3). Thus, an increased ILK expression was associated withsmaller LVDA as well as LVSA in female rats. However, a similar result was notobserved in male rats.

Bottom Line: MI size was similar between genders.MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders.Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-graduação em Ciências da Reabilitação, Universidade Nove de Julho, São Paulo, SP, Brazil.

ABSTRACT

Background: Gender can influence post-infarction cardiac remodeling.

Objective: To evaluate whether gender influences left ventricular (LV) remodeling and integrin-linked kinase (ILK) after myocardial infarction (MI).

Methods: Female and male Wistar rats were assigned to one of three groups: sham, moderate MI (size: 20-39% of LV area), and large MI (size: ≥40% of LV area). MI was induced by coronary occlusion, and echocardiographic analysis was performed after six weeks to evaluate MI size as well as LV morphology and function. Real-time RT-PCR and Western blot were used to quantify ILK in the myocardium.

Results: MI size was similar between genders. MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders. Female rats with large MI showed a lower diastolic and systolic dilatation than the respective male rats; however, LV dysfunction was similar between genders. Gene and protein levels of ILK were increased in female rats with moderate and large infarctions, but only male rats with large infarctions showed an altered ILK mRNA level. A negative linear correlation was evident between LV dimensions and ILK expression in female rats with large MI.

Conclusions: Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

Show MeSH
Related in: MedlinePlus