Limits...
Gender-based differences in cardiac remodeling and ILK expression after myocardial infarction.

Sofia RR, Serra AJ, Silva JA, Antonio EL, Manchini MT, Oliveira FA, Teixeira VP, Tucci PJ - Arq. Bras. Cardiol. (2014)

Bottom Line: MI size was similar between genders.MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders.Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-graduação em Ciências da Reabilitação, Universidade Nove de Julho, São Paulo, SP, Brazil.

ABSTRACT

Background: Gender can influence post-infarction cardiac remodeling.

Objective: To evaluate whether gender influences left ventricular (LV) remodeling and integrin-linked kinase (ILK) after myocardial infarction (MI).

Methods: Female and male Wistar rats were assigned to one of three groups: sham, moderate MI (size: 20-39% of LV area), and large MI (size: ≥40% of LV area). MI was induced by coronary occlusion, and echocardiographic analysis was performed after six weeks to evaluate MI size as well as LV morphology and function. Real-time RT-PCR and Western blot were used to quantify ILK in the myocardium.

Results: MI size was similar between genders. MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders. Female rats with large MI showed a lower diastolic and systolic dilatation than the respective male rats; however, LV dysfunction was similar between genders. Gene and protein levels of ILK were increased in female rats with moderate and large infarctions, but only male rats with large infarctions showed an altered ILK mRNA level. A negative linear correlation was evident between LV dimensions and ILK expression in female rats with large MI.

Conclusions: Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

Show MeSH

Related in: MedlinePlus

Quantification of (A) ILK mRNA and (B) ILK protein expression in the myocardiumof sham-operated and infarct-operated female and male rats, respectively. ILKlevels are plotted as a function of MI size. The sample size is shown into thebars. #p < 0.001 vs. respective sham; *p < 0.001 vs. respective moderateinfarction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150663&req=5

f01: Quantification of (A) ILK mRNA and (B) ILK protein expression in the myocardiumof sham-operated and infarct-operated female and male rats, respectively. ILKlevels are plotted as a function of MI size. The sample size is shown into thebars. #p < 0.001 vs. respective sham; *p < 0.001 vs. respective moderateinfarction.

Mentions: There was no significant difference in ILK expression between female and malesham-operated rats (Figure 1A). MIsignificantly increased ILK mRNA in female rats compared with the correspondingsham-operated female rats. ILK mRNA expression was higher in females with largeinfarcts than with moderate infarcts; however, this difference was not statisticallysignificant. In male rats, ILK levels were significantly increased in animals withlarge MI area. More importantly, gender significantly affected ILK mRNA expressionafter MI. Female rats with moderate and large MI size showed higher ILK levelscompared with male rats with moderate and large MI size. In Figure 1B, ILK protein levels examined by Western blot are shown.As observed for the gene expression, gender did not affect ILK content in themyocardial tissue of sham-operated groups. However, ILK protein levels wereupregulated as a function of MI size in females. On the other hand, ILK content wasnot altered in the myocardial tissue of infarcted male rats. Remarkably, given that asignificantly higher amount of ILK protein was found in female compared with malerats, gender determined differential expression of ILK after MI.


Gender-based differences in cardiac remodeling and ILK expression after myocardial infarction.

Sofia RR, Serra AJ, Silva JA, Antonio EL, Manchini MT, Oliveira FA, Teixeira VP, Tucci PJ - Arq. Bras. Cardiol. (2014)

Quantification of (A) ILK mRNA and (B) ILK protein expression in the myocardiumof sham-operated and infarct-operated female and male rats, respectively. ILKlevels are plotted as a function of MI size. The sample size is shown into thebars. #p < 0.001 vs. respective sham; *p < 0.001 vs. respective moderateinfarction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150663&req=5

f01: Quantification of (A) ILK mRNA and (B) ILK protein expression in the myocardiumof sham-operated and infarct-operated female and male rats, respectively. ILKlevels are plotted as a function of MI size. The sample size is shown into thebars. #p < 0.001 vs. respective sham; *p < 0.001 vs. respective moderateinfarction.
Mentions: There was no significant difference in ILK expression between female and malesham-operated rats (Figure 1A). MIsignificantly increased ILK mRNA in female rats compared with the correspondingsham-operated female rats. ILK mRNA expression was higher in females with largeinfarcts than with moderate infarcts; however, this difference was not statisticallysignificant. In male rats, ILK levels were significantly increased in animals withlarge MI area. More importantly, gender significantly affected ILK mRNA expressionafter MI. Female rats with moderate and large MI size showed higher ILK levelscompared with male rats with moderate and large MI size. In Figure 1B, ILK protein levels examined by Western blot are shown.As observed for the gene expression, gender did not affect ILK content in themyocardial tissue of sham-operated groups. However, ILK protein levels wereupregulated as a function of MI size in females. On the other hand, ILK content wasnot altered in the myocardial tissue of infarcted male rats. Remarkably, given that asignificantly higher amount of ILK protein was found in female compared with malerats, gender determined differential expression of ILK after MI.

Bottom Line: MI size was similar between genders.MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders.Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-graduação em Ciências da Reabilitação, Universidade Nove de Julho, São Paulo, SP, Brazil.

ABSTRACT

Background: Gender can influence post-infarction cardiac remodeling.

Objective: To evaluate whether gender influences left ventricular (LV) remodeling and integrin-linked kinase (ILK) after myocardial infarction (MI).

Methods: Female and male Wistar rats were assigned to one of three groups: sham, moderate MI (size: 20-39% of LV area), and large MI (size: ≥40% of LV area). MI was induced by coronary occlusion, and echocardiographic analysis was performed after six weeks to evaluate MI size as well as LV morphology and function. Real-time RT-PCR and Western blot were used to quantify ILK in the myocardium.

Results: MI size was similar between genders. MI resulted in systolic dysfunction and enlargement of end-diastolic as well as end-systolic dimension of LV as a function of necrotic area size in both genders. Female rats with large MI showed a lower diastolic and systolic dilatation than the respective male rats; however, LV dysfunction was similar between genders. Gene and protein levels of ILK were increased in female rats with moderate and large infarctions, but only male rats with large infarctions showed an altered ILK mRNA level. A negative linear correlation was evident between LV dimensions and ILK expression in female rats with large MI.

Conclusions: Post-MI ILK expression is altered in a gender-specific manner, and higher ILK levels found in females may be sufficient to improve LV geometry but not LV function.

Show MeSH
Related in: MedlinePlus