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Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus

The effect of EELU treatment on morphological changes assessed by histological examination of the renal cortex of rats by H&E stain (×100). Representative histological images (A) sham group; regular renal tissue with glomeruli (arrow) and tubuli (☆). (B) RIR group, cellular vacuolisation (arrowhead), congestion (arrow), and necrosis (☆); (C) EELU 200 mg/kg, reduced congestion (arrow); and (D) EELU 400 mg/kg showing reduced glomeruli necrosis (arrow) and tubular dilation (☆).
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f0015: The effect of EELU treatment on morphological changes assessed by histological examination of the renal cortex of rats by H&E stain (×100). Representative histological images (A) sham group; regular renal tissue with glomeruli (arrow) and tubuli (☆). (B) RIR group, cellular vacuolisation (arrowhead), congestion (arrow), and necrosis (☆); (C) EELU 200 mg/kg, reduced congestion (arrow); and (D) EELU 400 mg/kg showing reduced glomeruli necrosis (arrow) and tubular dilation (☆).

Mentions: Histological evaluation of the kidney in group I showed regular morphology of renal parenchyma with well-defined glomeruli and tubuli. Rats in group II showed severe (>50% area of the kidney) vascular congestion, tubular cell necrosis, cytoplasmic vacuolization and nuclear pyknosis. In groups III and IV, despite the presence of mild (0–25% area of the kidney) vasocongestion and tubular cell necrosis, the glomeruli maintained a better morphology than in group II (Fig. 3).


Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

The effect of EELU treatment on morphological changes assessed by histological examination of the renal cortex of rats by H&E stain (×100). Representative histological images (A) sham group; regular renal tissue with glomeruli (arrow) and tubuli (☆). (B) RIR group, cellular vacuolisation (arrowhead), congestion (arrow), and necrosis (☆); (C) EELU 200 mg/kg, reduced congestion (arrow); and (D) EELU 400 mg/kg showing reduced glomeruli necrosis (arrow) and tubular dilation (☆).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150603&req=5

f0015: The effect of EELU treatment on morphological changes assessed by histological examination of the renal cortex of rats by H&E stain (×100). Representative histological images (A) sham group; regular renal tissue with glomeruli (arrow) and tubuli (☆). (B) RIR group, cellular vacuolisation (arrowhead), congestion (arrow), and necrosis (☆); (C) EELU 200 mg/kg, reduced congestion (arrow); and (D) EELU 400 mg/kg showing reduced glomeruli necrosis (arrow) and tubular dilation (☆).
Mentions: Histological evaluation of the kidney in group I showed regular morphology of renal parenchyma with well-defined glomeruli and tubuli. Rats in group II showed severe (>50% area of the kidney) vascular congestion, tubular cell necrosis, cytoplasmic vacuolization and nuclear pyknosis. In groups III and IV, despite the presence of mild (0–25% area of the kidney) vasocongestion and tubular cell necrosis, the glomeruli maintained a better morphology than in group II (Fig. 3).

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus