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Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus

Effect of EELU on cellular necrosis and viability detected in renal cells by flow cytometry using the PI method; PI fluorescence intensity was measured using FL-1. Representative dot-plot images from four experimental groups: (A) sham, (B) RIR, (C) EELU 200 mg/kg, and (D) EELU 400 mg/kg. A histogram of cellular necrosis (E) and viability (F) were plotted; each bar represents the mean ± SEM of four experimental groups. ∗∗∗P < 0.001 vs. RIR group.
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f0010: Effect of EELU on cellular necrosis and viability detected in renal cells by flow cytometry using the PI method; PI fluorescence intensity was measured using FL-1. Representative dot-plot images from four experimental groups: (A) sham, (B) RIR, (C) EELU 200 mg/kg, and (D) EELU 400 mg/kg. A histogram of cellular necrosis (E) and viability (F) were plotted; each bar represents the mean ± SEM of four experimental groups. ∗∗∗P < 0.001 vs. RIR group.

Mentions: Cellular necrosis in group II was increased significantly (P < 0.001) whereas groups III and IV had a significant (both P < 0.001) decrease in cellular necrosis. The viability of renal cells was significantly (P < 0.001) decreased in group II and significantly (P < 0.001) greater in groups III and IV than in group II (Fig. 2).


Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

Effect of EELU on cellular necrosis and viability detected in renal cells by flow cytometry using the PI method; PI fluorescence intensity was measured using FL-1. Representative dot-plot images from four experimental groups: (A) sham, (B) RIR, (C) EELU 200 mg/kg, and (D) EELU 400 mg/kg. A histogram of cellular necrosis (E) and viability (F) were plotted; each bar represents the mean ± SEM of four experimental groups. ∗∗∗P < 0.001 vs. RIR group.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150603&req=5

f0010: Effect of EELU on cellular necrosis and viability detected in renal cells by flow cytometry using the PI method; PI fluorescence intensity was measured using FL-1. Representative dot-plot images from four experimental groups: (A) sham, (B) RIR, (C) EELU 200 mg/kg, and (D) EELU 400 mg/kg. A histogram of cellular necrosis (E) and viability (F) were plotted; each bar represents the mean ± SEM of four experimental groups. ∗∗∗P < 0.001 vs. RIR group.
Mentions: Cellular necrosis in group II was increased significantly (P < 0.001) whereas groups III and IV had a significant (both P < 0.001) decrease in cellular necrosis. The viability of renal cells was significantly (P < 0.001) decreased in group II and significantly (P < 0.001) greater in groups III and IV than in group II (Fig. 2).

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus