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Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus

Effects of EELU on DNA fragmentation in RIR-induced injury in rats: (A) sham, (B) RIR, (C) EELU 200 mg/kg, (D) EELU 400 mg/kg groups.
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f0005: Effects of EELU on DNA fragmentation in RIR-induced injury in rats: (A) sham, (B) RIR, (C) EELU 200 mg/kg, (D) EELU 400 mg/kg groups.

Mentions: DNA fragmentation in renal tissue was analysed with agarose-gel electrophoresis. In group II there was intra-nucleosomal DNA damage, in the form of typical fragmentation laddering, which indicates cell apoptosis (Fig. 1, lane B), whereas there was less DNA fragmentation in groups III and IV (Fig. 1, lanes C and D, respectively) suggesting anti-apoptotic activity preventing cell death during RIR-induced renal hypertension.


Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia-reperfusion injury in rats.

Ghule AE, Jadhav SS, Bodhankar SL - Arab J Urol (2011)

Effects of EELU on DNA fragmentation in RIR-induced injury in rats: (A) sham, (B) RIR, (C) EELU 200 mg/kg, (D) EELU 400 mg/kg groups.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150603&req=5

f0005: Effects of EELU on DNA fragmentation in RIR-induced injury in rats: (A) sham, (B) RIR, (C) EELU 200 mg/kg, (D) EELU 400 mg/kg groups.
Mentions: DNA fragmentation in renal tissue was analysed with agarose-gel electrophoresis. In group II there was intra-nucleosomal DNA damage, in the form of typical fragmentation laddering, which indicates cell apoptosis (Fig. 1, lane B), whereas there was less DNA fragmentation in groups III and IV (Fig. 1, lanes C and D, respectively) suggesting anti-apoptotic activity preventing cell death during RIR-induced renal hypertension.

Bottom Line: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury.The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation.We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.

ABSTRACT

Objective: To evaluate an ethanolic extract of seeds of Linum usitatissimum (Linn.) (EELU) for its renoprotective role in rats through its antihypertensive effect and conservation of biological oxidation enzymes.

Materials and methods: Male Wistar rats (200-250 g) underwent uninephrectomy on day 0; after 2 weeks of recovery, the nephrectomised rats were divided into four groups of eight each: (I) sham (II); renal ischaemia reperfusion (RIR); (III) RIR + EELU 200 mg/kg; and (IV) RIR + EELU 400 mg/kg. In group II, III and IV the renal artery was occluded for 45 min and reperfused for 4 weeks; the sham group did not undergo RIR.

Results: EELU (400 mg/kg) significantly decreased the haemodynamic changes after 4 weeks of RIR injury. EELU treatment significantly restored the levels of renal endogenous antioxidant enzymes and membrane-bound enzymes. EELU 400 mg/kg restored the levels of blood urea nitrogen and serum creatinine. EELU also decreased the levels of tumour necrosis factor-α and myeloperoxidase activity. A flow-cytometric study confirmed a significant decrease in cellular necrosis and increase in viability after RIR in EELU-treated rats. The anti-apoptotic role of EELU was evident from the decrease in DNA fragmentation. Renal tissue damage as assessed by histopathology was decreased in groups III and IV (200 and 400 mg/kg EELU).

Conclusion: We conclude that EELU protected the kidney against RIR-induced renal injury, probably by inhibiting reactive oxygen species that have a causal role in such cases. It also inhibits apoptotic cell death and inflammation, and improves haemodynamic changes.

No MeSH data available.


Related in: MedlinePlus