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The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study.

Harraz AM, Lin G, Banie L, Wang G, Shindel AW, Huang YC, Fandel TM, Garcia M, Lue TF, Lin CS - Arab J Urol (2011)

Bottom Line: However, the bladder weight was significantly greater in the ADSC-treated group.On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

View Article: PubMed Central - PubMed

Affiliation: Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, CA 94143-0738, USA ; Urology and Nephrology Centre, Mansoura University, Egypt.

ABSTRACT

Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function.

Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining.

Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.

Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

No MeSH data available.


Masson trichrome staining in the AI + ADSC group and AI-only group.
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f0020: Masson trichrome staining in the AI + ADSC group and AI-only group.

Mentions: The anastomosed ileo-bladder tissue in each AI + ADSC and AI-only rat was assessed for muscle vs matrix content at 8 weeks after surgery by trichrome stain (Fig. 4). There was a trend to higher muscle vs. muscle content in the AI + ADSC than in the AI-only group, but the difference was not statistically significant (P = 0.2). Nevertheless, the wet weight of the bladder in the AI + ADSC group was significantly higher than in AI-only group (Table 1). The elastin content was also significantly greater in the AI + ADSC than in the AI-only group (Fig. 5).


The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study.

Harraz AM, Lin G, Banie L, Wang G, Shindel AW, Huang YC, Fandel TM, Garcia M, Lue TF, Lin CS - Arab J Urol (2011)

Masson trichrome staining in the AI + ADSC group and AI-only group.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150570&req=5

f0020: Masson trichrome staining in the AI + ADSC group and AI-only group.
Mentions: The anastomosed ileo-bladder tissue in each AI + ADSC and AI-only rat was assessed for muscle vs matrix content at 8 weeks after surgery by trichrome stain (Fig. 4). There was a trend to higher muscle vs. muscle content in the AI + ADSC than in the AI-only group, but the difference was not statistically significant (P = 0.2). Nevertheless, the wet weight of the bladder in the AI + ADSC group was significantly higher than in AI-only group (Table 1). The elastin content was also significantly greater in the AI + ADSC than in the AI-only group (Fig. 5).

Bottom Line: However, the bladder weight was significantly greater in the ADSC-treated group.On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

View Article: PubMed Central - PubMed

Affiliation: Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, CA 94143-0738, USA ; Urology and Nephrology Centre, Mansoura University, Egypt.

ABSTRACT

Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function.

Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining.

Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.

Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

No MeSH data available.