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The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study.

Harraz AM, Lin G, Banie L, Wang G, Shindel AW, Huang YC, Fandel TM, Garcia M, Lue TF, Lin CS - Arab J Urol (2011)

Bottom Line: However, the bladder weight was significantly greater in the ADSC-treated group.On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

View Article: PubMed Central - PubMed

Affiliation: Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, CA 94143-0738, USA ; Urology and Nephrology Centre, Mansoura University, Egypt.

ABSTRACT

Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function.

Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining.

Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.

Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

No MeSH data available.


(A) 0.5 cm of terminal ileum is isolated (arrows), (B) re-continuity of ileum is restored, (C) the ileal segment is detubularized and anastomosed to the bladder after its dome has been excised (arrow heads).
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f0005: (A) 0.5 cm of terminal ileum is isolated (arrows), (B) re-continuity of ileum is restored, (C) the ileal segment is detubularized and anastomosed to the bladder after its dome has been excised (arrow heads).

Mentions: Several different surgical procedures for AI were tested before selecting the one described here. Rats were fasted the night before surgery to reduce the bowel content. Under 2% isoflurane anaesthesia, a midline abdominal incision was made to expose the ileum and urinary bladder. In the sham group no further treatment was done except for closing the wound. In the other two groups the rats were further treated as follows. Briefly, 1 cm of the terminal ileum was isolated and transected on both sides, while leaving the mesentery intact. The intestine was then re-anastomosed, and the isolated ileal segment was anastomosed to the bladder after excising its dome (Fig. 1). Afterward, 1 mL of PBS and ADSCs were injected into the submucosal layer of the ileal segment in the AI-only group and the AI + ADSC group, respectively. The wound was then closed in two layers with absorbable suture.


The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study.

Harraz AM, Lin G, Banie L, Wang G, Shindel AW, Huang YC, Fandel TM, Garcia M, Lue TF, Lin CS - Arab J Urol (2011)

(A) 0.5 cm of terminal ileum is isolated (arrows), (B) re-continuity of ileum is restored, (C) the ileal segment is detubularized and anastomosed to the bladder after its dome has been excised (arrow heads).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150570&req=5

f0005: (A) 0.5 cm of terminal ileum is isolated (arrows), (B) re-continuity of ileum is restored, (C) the ileal segment is detubularized and anastomosed to the bladder after its dome has been excised (arrow heads).
Mentions: Several different surgical procedures for AI were tested before selecting the one described here. Rats were fasted the night before surgery to reduce the bowel content. Under 2% isoflurane anaesthesia, a midline abdominal incision was made to expose the ileum and urinary bladder. In the sham group no further treatment was done except for closing the wound. In the other two groups the rats were further treated as follows. Briefly, 1 cm of the terminal ileum was isolated and transected on both sides, while leaving the mesentery intact. The intestine was then re-anastomosed, and the isolated ileal segment was anastomosed to the bladder after excising its dome (Fig. 1). Afterward, 1 mL of PBS and ADSCs were injected into the submucosal layer of the ileal segment in the AI-only group and the AI + ADSC group, respectively. The wound was then closed in two layers with absorbable suture.

Bottom Line: However, the bladder weight was significantly greater in the ADSC-treated group.On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

View Article: PubMed Central - PubMed

Affiliation: Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, CA 94143-0738, USA ; Urology and Nephrology Centre, Mansoura University, Egypt.

ABSTRACT

Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function.

Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining.

Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells.

Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

No MeSH data available.