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A proteomics analysis to evaluate cytotoxicity in NRK-52E cells caused by unmodified Nano-Fe₃O₄.

Lin YR, Kuo CJ, Lin HY, Wu CJ, Liang SS - ScientificWorldJournal (2014)

Bottom Line: Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins.From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins.In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Fooyin University, 151 Jinxue Road, Kaohsiung 83102, Taiwan.

ABSTRACT
We synthesized unmodified Fe₃O₄ nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe₃O₄ NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins. From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins. In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins. We suggested that, in the indigenous cellular environment, Fe₃O₄ NPs treatment induced an antagonistic effect for cell lines to go to which avoids apoptosis.

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Related in: MedlinePlus

TEM images of Fe3O4 NPs with different sizes as follows: (a) 20 nm, (b) 50 nm, and (c) 100 nm scale bars.
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Related In: Results  -  Collection


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fig1: TEM images of Fe3O4 NPs with different sizes as follows: (a) 20 nm, (b) 50 nm, and (c) 100 nm scale bars.

Mentions: Bare and unmodified Fe3O4 NPs were synthesized by hydrothermal precipitation and NPs were fabricated by chemical method to have a uniform particle size. In Figure 1, TEM images showed that we took three photos of Fe3O4 NPs with 20 nm, 50 nm, and 100 nm scale bars. It is considered that NPs with a higher surface area are easier to aggregate with other NPs; therefore through the aggregation in Fe3O4 NPs there were Fe3O4 particles generated above 100 nm if no additional protective agent was modified onto Fe3O4 (Figure 1(c)). We considered that Fe3O4 NPs with dispersive diameter sizes are suitable for cytotoxicity evaluation due to the fact that NPs found in the environment have random sizes.


A proteomics analysis to evaluate cytotoxicity in NRK-52E cells caused by unmodified Nano-Fe₃O₄.

Lin YR, Kuo CJ, Lin HY, Wu CJ, Liang SS - ScientificWorldJournal (2014)

TEM images of Fe3O4 NPs with different sizes as follows: (a) 20 nm, (b) 50 nm, and (c) 100 nm scale bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150542&req=5

fig1: TEM images of Fe3O4 NPs with different sizes as follows: (a) 20 nm, (b) 50 nm, and (c) 100 nm scale bars.
Mentions: Bare and unmodified Fe3O4 NPs were synthesized by hydrothermal precipitation and NPs were fabricated by chemical method to have a uniform particle size. In Figure 1, TEM images showed that we took three photos of Fe3O4 NPs with 20 nm, 50 nm, and 100 nm scale bars. It is considered that NPs with a higher surface area are easier to aggregate with other NPs; therefore through the aggregation in Fe3O4 NPs there were Fe3O4 particles generated above 100 nm if no additional protective agent was modified onto Fe3O4 (Figure 1(c)). We considered that Fe3O4 NPs with dispersive diameter sizes are suitable for cytotoxicity evaluation due to the fact that NPs found in the environment have random sizes.

Bottom Line: Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins.From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins.In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Fooyin University, 151 Jinxue Road, Kaohsiung 83102, Taiwan.

ABSTRACT
We synthesized unmodified Fe₃O₄ nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe₃O₄ NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins. From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins. In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins. We suggested that, in the indigenous cellular environment, Fe₃O₄ NPs treatment induced an antagonistic effect for cell lines to go to which avoids apoptosis.

Show MeSH
Related in: MedlinePlus