Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.
Bottom Line: The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities.A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma.Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.
Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.Show MeSH
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Mentions: In the CA1 area, the Kir3.1, Kir3.2 and Kir3.3 subunits showed a qualitatively similar pattern of labelling (Fig. 6). The SO, SP and the proximal part of the SR were weakly labelled and the labelling intensity increased gradually towards the distal SR and SLM. Out of the three subunits, the overall intensity was highest for the Kir3.2 and weakest for the Kir3.3 subunit. Our LM observations are in line with those of previous reports (Ponce et al., 1996; Koyrakh et al., 2005; Kulik et al., 2006; Fernandez-Alacid et al., 2011).
Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.