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Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.

Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z - Eur. J. Neurosci. (2014)

Bottom Line: The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities.A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma.Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.

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Distributions of three different Kir3 subunits in the CA1 region of rat hippocampus. (A) Immunofluorescent reaction for the Kir3.1 subunit shows a modest labelling in SO and in the proximal part of SR with a gradual increase towards the distal part of the SR and SLM. (B) Similar labelling pattern is observed for the Kir3.2 subunit, with the most intense immunolabelling in the distal part of the SR and SLM. (C) The CA1 area shows weak immunoreactivity for the Kir3.3 subunit, but the intensity of the signal increases toward the distal SR and SLM. Scale bars, 100 μm (A–C).
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fig06: Distributions of three different Kir3 subunits in the CA1 region of rat hippocampus. (A) Immunofluorescent reaction for the Kir3.1 subunit shows a modest labelling in SO and in the proximal part of SR with a gradual increase towards the distal part of the SR and SLM. (B) Similar labelling pattern is observed for the Kir3.2 subunit, with the most intense immunolabelling in the distal part of the SR and SLM. (C) The CA1 area shows weak immunoreactivity for the Kir3.3 subunit, but the intensity of the signal increases toward the distal SR and SLM. Scale bars, 100 μm (A–C).

Mentions: In the CA1 area, the Kir3.1, Kir3.2 and Kir3.3 subunits showed a qualitatively similar pattern of labelling (Fig. 6). The SO, SP and the proximal part of the SR were weakly labelled and the labelling intensity increased gradually towards the distal SR and SLM. Out of the three subunits, the overall intensity was highest for the Kir3.2 and weakest for the Kir3.3 subunit. Our LM observations are in line with those of previous reports (Ponce et al., 1996; Koyrakh et al., 2005; Kulik et al., 2006; Fernandez-Alacid et al., 2011).


Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.

Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z - Eur. J. Neurosci. (2014)

Distributions of three different Kir3 subunits in the CA1 region of rat hippocampus. (A) Immunofluorescent reaction for the Kir3.1 subunit shows a modest labelling in SO and in the proximal part of SR with a gradual increase towards the distal part of the SR and SLM. (B) Similar labelling pattern is observed for the Kir3.2 subunit, with the most intense immunolabelling in the distal part of the SR and SLM. (C) The CA1 area shows weak immunoreactivity for the Kir3.3 subunit, but the intensity of the signal increases toward the distal SR and SLM. Scale bars, 100 μm (A–C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150533&req=5

fig06: Distributions of three different Kir3 subunits in the CA1 region of rat hippocampus. (A) Immunofluorescent reaction for the Kir3.1 subunit shows a modest labelling in SO and in the proximal part of SR with a gradual increase towards the distal part of the SR and SLM. (B) Similar labelling pattern is observed for the Kir3.2 subunit, with the most intense immunolabelling in the distal part of the SR and SLM. (C) The CA1 area shows weak immunoreactivity for the Kir3.3 subunit, but the intensity of the signal increases toward the distal SR and SLM. Scale bars, 100 μm (A–C).
Mentions: In the CA1 area, the Kir3.1, Kir3.2 and Kir3.3 subunits showed a qualitatively similar pattern of labelling (Fig. 6). The SO, SP and the proximal part of the SR were weakly labelled and the labelling intensity increased gradually towards the distal SR and SLM. Out of the three subunits, the overall intensity was highest for the Kir3.2 and weakest for the Kir3.3 subunit. Our LM observations are in line with those of previous reports (Ponce et al., 1996; Koyrakh et al., 2005; Kulik et al., 2006; Fernandez-Alacid et al., 2011).

Bottom Line: The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities.A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma.Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.

Show MeSH
Related in: MedlinePlus