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Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.

Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z - Eur. J. Neurosci. (2014)

Bottom Line: The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities.A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma.Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.

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Densities of gold particles labelling the Kv2.1 subunit in different subcellular compartments of CA1 PCs. (A) Bar graphs show the Kv2.1 subunit densities (mean ± SD) in different axo-somato-dendritic compartments. Significant densities of gold particles labelling the Kv2.1 subunit (*) are found on the somata and proximal apical dendrites (ApDendr) of CA1 PCs (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats). (B) Gold particle density for the Kv2.1 subunit on the AISs of CA1 PCs was significantly different from the background (BG; anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats). These data are obtained from double-labelling experiments for the Kv2.1 and Kv1.1 subunits. Gold particle densities for the Kv2.1 subunit on PC somata was similar in single- and double-labelling experiments (cf. A and B). AxTerm, axon terminal; OblDendr, oblique dendrite; Tuft Dendr, tuft dendrite; prox., proximal; mid., middle; dist., distal.
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fig05: Densities of gold particles labelling the Kv2.1 subunit in different subcellular compartments of CA1 PCs. (A) Bar graphs show the Kv2.1 subunit densities (mean ± SD) in different axo-somato-dendritic compartments. Significant densities of gold particles labelling the Kv2.1 subunit (*) are found on the somata and proximal apical dendrites (ApDendr) of CA1 PCs (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats). (B) Gold particle density for the Kv2.1 subunit on the AISs of CA1 PCs was significantly different from the background (BG; anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats). These data are obtained from double-labelling experiments for the Kv2.1 and Kv1.1 subunits. Gold particle densities for the Kv2.1 subunit on PC somata was similar in single- and double-labelling experiments (cf. A and B). AxTerm, axon terminal; OblDendr, oblique dendrite; Tuft Dendr, tuft dendrite; prox., proximal; mid., middle; dist., distal.

Mentions: Next we performed a quantitative comparison of the Kv2.1 densities in 18 axo-somato-dendritic compartments of CA1 PCs (Table 2). The densities of immunogold particles for the Kv2.1 subunit in the somato-dendritic compartments and axon terminals were calculated from single-labelling experiments. Somata and proximal apical dendrites contained high densities of gold particles, which were significantly (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats; Fig. 5A) higher than background. The densities of the Kv2.1 subunit in apical dendrites in the middle and distal SR, SLM tuft dendrites, oblique dendrites, dendritic spines, and axon terminals were not significantly different from the nonspecific background labelling (anova, P < 0.001; Dunnett's post hoc test, P > 0.26; n = 3 rats). The density of the Kv2.1 subunit in AISs was calculated from double-labelling experiments with the Kv1.1 subunit. The strength of the Kv2.1 labelling of somata [11.4 ± 3.8 gold particles per μm2 (gold/μm2)] in these double-labelling experiments was very similar to that found in single-labelling reactions (P = 0.66, unpaired Student's t-test). AISs contained, on average, 11.5 ± 1.8 gold/μm2, which did not differ significantly from the gold particle content of somata (P = 0.97, unpaired Student's t-test), but was significantly above the background (anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats; Fig. 5B).


Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.

Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z - Eur. J. Neurosci. (2014)

Densities of gold particles labelling the Kv2.1 subunit in different subcellular compartments of CA1 PCs. (A) Bar graphs show the Kv2.1 subunit densities (mean ± SD) in different axo-somato-dendritic compartments. Significant densities of gold particles labelling the Kv2.1 subunit (*) are found on the somata and proximal apical dendrites (ApDendr) of CA1 PCs (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats). (B) Gold particle density for the Kv2.1 subunit on the AISs of CA1 PCs was significantly different from the background (BG; anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats). These data are obtained from double-labelling experiments for the Kv2.1 and Kv1.1 subunits. Gold particle densities for the Kv2.1 subunit on PC somata was similar in single- and double-labelling experiments (cf. A and B). AxTerm, axon terminal; OblDendr, oblique dendrite; Tuft Dendr, tuft dendrite; prox., proximal; mid., middle; dist., distal.
© Copyright Policy - open-access
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fig05: Densities of gold particles labelling the Kv2.1 subunit in different subcellular compartments of CA1 PCs. (A) Bar graphs show the Kv2.1 subunit densities (mean ± SD) in different axo-somato-dendritic compartments. Significant densities of gold particles labelling the Kv2.1 subunit (*) are found on the somata and proximal apical dendrites (ApDendr) of CA1 PCs (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats). (B) Gold particle density for the Kv2.1 subunit on the AISs of CA1 PCs was significantly different from the background (BG; anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats). These data are obtained from double-labelling experiments for the Kv2.1 and Kv1.1 subunits. Gold particle densities for the Kv2.1 subunit on PC somata was similar in single- and double-labelling experiments (cf. A and B). AxTerm, axon terminal; OblDendr, oblique dendrite; Tuft Dendr, tuft dendrite; prox., proximal; mid., middle; dist., distal.
Mentions: Next we performed a quantitative comparison of the Kv2.1 densities in 18 axo-somato-dendritic compartments of CA1 PCs (Table 2). The densities of immunogold particles for the Kv2.1 subunit in the somato-dendritic compartments and axon terminals were calculated from single-labelling experiments. Somata and proximal apical dendrites contained high densities of gold particles, which were significantly (anova, P < 0.001; Dunnett's post hoc test, P < 0.001; n = 3 rats; Fig. 5A) higher than background. The densities of the Kv2.1 subunit in apical dendrites in the middle and distal SR, SLM tuft dendrites, oblique dendrites, dendritic spines, and axon terminals were not significantly different from the nonspecific background labelling (anova, P < 0.001; Dunnett's post hoc test, P > 0.26; n = 3 rats). The density of the Kv2.1 subunit in AISs was calculated from double-labelling experiments with the Kv1.1 subunit. The strength of the Kv2.1 labelling of somata [11.4 ± 3.8 gold particles per μm2 (gold/μm2)] in these double-labelling experiments was very similar to that found in single-labelling reactions (P = 0.66, unpaired Student's t-test). AISs contained, on average, 11.5 ± 1.8 gold/μm2, which did not differ significantly from the gold particle content of somata (P = 0.97, unpaired Student's t-test), but was significantly above the background (anova, P < 0.01; Dunnett's post hoc test, P < 0.01; n = 3 rats; Fig. 5B).

Bottom Line: The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities.A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma.Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Budapest, Hungary.

Show MeSH
Related in: MedlinePlus