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Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus

Effects of electroacupuncture on adipose tissue macrophage cell marker (F4/80) and inflammation mRNA levels of chemokines in animals with dietary obesity. Histomorphometrical analysis of adipose tissue sections stained with F4/80 (original magnification ×40) (a) and mRNA quantitated in rats adipose tissue by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate F4/80 positive cells. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Scale bars, 100 μm.
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fig4: Effects of electroacupuncture on adipose tissue macrophage cell marker (F4/80) and inflammation mRNA levels of chemokines in animals with dietary obesity. Histomorphometrical analysis of adipose tissue sections stained with F4/80 (original magnification ×40) (a) and mRNA quantitated in rats adipose tissue by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate F4/80 positive cells. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Scale bars, 100 μm.

Mentions: Increased adiposity is associated with increased infiltration of macrophages into the adipose tissue. Therefore, we determined the effect of EA on inflammatory responses in epididymal adipose tissue. Consistent with previous result, staining of adipose tissue with antibodies to the macrophage marker F4/80 (Figure 4(a)) showed a high frequency of macrophages in the adipose tissue of HFD rats, which was confirmed by gene expression analysis of F4/80 with real-time PCR (Figure 4(b)). The number of F4/80 positive cells in adipose tissue was significantly different among HFD and HFD with EA treatment groups. EA treatment in HFD rats significantly decreased adipose tissue TNF-α (Figure 4(c)), MCP-1 (Figure 4(d)), CD68 (Figure 4(e)), and IL-6 (Figure 4(f)) mRNA levels compared to those in HFD controls. In the adipose tissue of rats treated with EA, however, the number of macrophages was decreased concomitant with decreased expression of F4/80 and CD68 mRNA. Consistent with the increased macrophage content, the expression of MCP-1, a chemokine involved in macrophage recruitment, was upregulated nearly threefold by the HFD.


Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Effects of electroacupuncture on adipose tissue macrophage cell marker (F4/80) and inflammation mRNA levels of chemokines in animals with dietary obesity. Histomorphometrical analysis of adipose tissue sections stained with F4/80 (original magnification ×40) (a) and mRNA quantitated in rats adipose tissue by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate F4/80 positive cells. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Scale bars, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4150518&req=5

fig4: Effects of electroacupuncture on adipose tissue macrophage cell marker (F4/80) and inflammation mRNA levels of chemokines in animals with dietary obesity. Histomorphometrical analysis of adipose tissue sections stained with F4/80 (original magnification ×40) (a) and mRNA quantitated in rats adipose tissue by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate F4/80 positive cells. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Scale bars, 100 μm.
Mentions: Increased adiposity is associated with increased infiltration of macrophages into the adipose tissue. Therefore, we determined the effect of EA on inflammatory responses in epididymal adipose tissue. Consistent with previous result, staining of adipose tissue with antibodies to the macrophage marker F4/80 (Figure 4(a)) showed a high frequency of macrophages in the adipose tissue of HFD rats, which was confirmed by gene expression analysis of F4/80 with real-time PCR (Figure 4(b)). The number of F4/80 positive cells in adipose tissue was significantly different among HFD and HFD with EA treatment groups. EA treatment in HFD rats significantly decreased adipose tissue TNF-α (Figure 4(c)), MCP-1 (Figure 4(d)), CD68 (Figure 4(e)), and IL-6 (Figure 4(f)) mRNA levels compared to those in HFD controls. In the adipose tissue of rats treated with EA, however, the number of macrophages was decreased concomitant with decreased expression of F4/80 and CD68 mRNA. Consistent with the increased macrophage content, the expression of MCP-1, a chemokine involved in macrophage recruitment, was upregulated nearly threefold by the HFD.

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus