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Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus

Effects of electroacupuncture on both neutrophil and macrophage activation markers in adipose tissue and cytokines levels altered in animals with dietary obesity. Neutrophil (a), CD11b+ (b) in adipose tissue, and serum TNF-α (c), IL-1 (d) and IL-6 (e) levels, in rats fed with either an HFD or a normal chow. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate neutrophil and CD11b+ positive cells, respectively. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
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fig3: Effects of electroacupuncture on both neutrophil and macrophage activation markers in adipose tissue and cytokines levels altered in animals with dietary obesity. Neutrophil (a), CD11b+ (b) in adipose tissue, and serum TNF-α (c), IL-1 (d) and IL-6 (e) levels, in rats fed with either an HFD or a normal chow. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate neutrophil and CD11b+ positive cells, respectively. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.

Mentions: Because obesity is associated with leukocyte accumulation in adipose tissue, which may be mediated by cytokines and contribute to adipose tissue inflammation, we examined effects of EA on neutrophil accumulation in adipose tissue. Compared to normal chow rats, HFD animals had increased levels of neutrophile (Figure 3(a)) and CD11b+ (activation of macrophage marker, Figure 3(b)) in adipose tissue. Compared to HFD group, HFD rats treated with intense EA have significantly different levels of neutrophil/macrophage cell markers. Based on the active roles of cytokines in inflammation, we examined the effect of EA on proinflammatory cytokines genes expression in adipose tissue. Compared to the lean control, obese HFD rats had increased levels of several cytokines, including TNF-α, IL-1, and IL-6, in adipose tissue (Figures 3(c)–3(e)). EA treated animals also had lower mRNA levels of TNF-α and IL-6 than HFD group.


Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Effects of electroacupuncture on both neutrophil and macrophage activation markers in adipose tissue and cytokines levels altered in animals with dietary obesity. Neutrophil (a), CD11b+ (b) in adipose tissue, and serum TNF-α (c), IL-1 (d) and IL-6 (e) levels, in rats fed with either an HFD or a normal chow. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate neutrophil and CD11b+ positive cells, respectively. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Effects of electroacupuncture on both neutrophil and macrophage activation markers in adipose tissue and cytokines levels altered in animals with dietary obesity. Neutrophil (a), CD11b+ (b) in adipose tissue, and serum TNF-α (c), IL-1 (d) and IL-6 (e) levels, in rats fed with either an HFD or a normal chow. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate neutrophil and CD11b+ positive cells, respectively. Results are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
Mentions: Because obesity is associated with leukocyte accumulation in adipose tissue, which may be mediated by cytokines and contribute to adipose tissue inflammation, we examined effects of EA on neutrophil accumulation in adipose tissue. Compared to normal chow rats, HFD animals had increased levels of neutrophile (Figure 3(a)) and CD11b+ (activation of macrophage marker, Figure 3(b)) in adipose tissue. Compared to HFD group, HFD rats treated with intense EA have significantly different levels of neutrophil/macrophage cell markers. Based on the active roles of cytokines in inflammation, we examined the effect of EA on proinflammatory cytokines genes expression in adipose tissue. Compared to the lean control, obese HFD rats had increased levels of several cytokines, including TNF-α, IL-1, and IL-6, in adipose tissue (Figures 3(c)–3(e)). EA treated animals also had lower mRNA levels of TNF-α and IL-6 than HFD group.

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus