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Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus

Effects of electroacupuncture on adipose tissue expression of lipogenic genes and serum lipid profiles. mRNA levels of selected lipogenic genes in adipose tissue after EA treatment were examined by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Values are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. SREBP1c: sterol response element binding protein 1c. ACC: acetyl-CoA carboxylase. FAS: fatty acid synthase. SCD-1: stearoyl-CoA desaturase-1. Original magnification ×40. Scale bars, 100 μm.
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fig2: Effects of electroacupuncture on adipose tissue expression of lipogenic genes and serum lipid profiles. mRNA levels of selected lipogenic genes in adipose tissue after EA treatment were examined by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Values are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. SREBP1c: sterol response element binding protein 1c. ACC: acetyl-CoA carboxylase. FAS: fatty acid synthase. SCD-1: stearoyl-CoA desaturase-1. Original magnification ×40. Scale bars, 100 μm.

Mentions: To elucidate the underlying mechanisms of the effects of EA, we used quantitative RT-PCR after 20 weeks of HFD feeding to examine EA-induced early changes in lipogenesis gene expression in adipose tissues (Figure 2). Intense treatment with EA significantly downregulated the mRNA levels of SREBP1c, FAS, ACC1, and SCD1, which are lipogenic enzymes in the adipose tissue, compared with the levels in the high fat vehicle group (Figures 2(a)–2(d)). The mRNA level of SREBP1c, the master regulator of fatty acid synthesis, was lower in the EA group than in the HFD group. Similarly, EA treatment decreased plasma lipid profiles, including total cholesterol, triglyceride, and free fatty acids (Figures 2(e)–2(g)) in HFD groups. On the other hand, there were marked differences in the immunohistochemical analysis that fatty acid transporter (CD36, Figure 2(h)) between the groups in epididymal white adipose tissue.


Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Effects of electroacupuncture on adipose tissue expression of lipogenic genes and serum lipid profiles. mRNA levels of selected lipogenic genes in adipose tissue after EA treatment were examined by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Values are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. SREBP1c: sterol response element binding protein 1c. ACC: acetyl-CoA carboxylase. FAS: fatty acid synthase. SCD-1: stearoyl-CoA desaturase-1. Original magnification ×40. Scale bars, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4150518&req=5

fig2: Effects of electroacupuncture on adipose tissue expression of lipogenic genes and serum lipid profiles. mRNA levels of selected lipogenic genes in adipose tissue after EA treatment were examined by quantitative reverse transcription polymerase chain reaction. Each result represented the experiment performed in triplicate assays in the different experimental groups. Values are expressed as means ± SEM. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. SREBP1c: sterol response element binding protein 1c. ACC: acetyl-CoA carboxylase. FAS: fatty acid synthase. SCD-1: stearoyl-CoA desaturase-1. Original magnification ×40. Scale bars, 100 μm.
Mentions: To elucidate the underlying mechanisms of the effects of EA, we used quantitative RT-PCR after 20 weeks of HFD feeding to examine EA-induced early changes in lipogenesis gene expression in adipose tissues (Figure 2). Intense treatment with EA significantly downregulated the mRNA levels of SREBP1c, FAS, ACC1, and SCD1, which are lipogenic enzymes in the adipose tissue, compared with the levels in the high fat vehicle group (Figures 2(a)–2(d)). The mRNA level of SREBP1c, the master regulator of fatty acid synthesis, was lower in the EA group than in the HFD group. Similarly, EA treatment decreased plasma lipid profiles, including total cholesterol, triglyceride, and free fatty acids (Figures 2(e)–2(g)) in HFD groups. On the other hand, there were marked differences in the immunohistochemical analysis that fatty acid transporter (CD36, Figure 2(h)) between the groups in epididymal white adipose tissue.

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus