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Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus

Effects of electroacupuncture on body weight, fat accumulation, and adipocyte hypertrophy in high fat diet rats. Animals with high fat diet-induced obesity were treated with EA once by daily for three times (T3) or seven times (T7) in the age of 20 weeks. Pair-feeding was conducted in three separate groups of obese rats to achieve the same body weight during this period; n = 5 rat/group. Flow chart of the study design (a), detailed view of the growth curve during the experimental period, body weight (b, left panel), and food intake was measured on a per-cage basis throughout the study and represents cumulative energy intake (b, right panel) and total adipocyte size expressed at the end of treatment (c). H&E staining showing a decrease in adipocyte size in EA treatment rats, compared with obese animals fed a HFD (e). Distribution of adipocyte sizes indicates a shift in the size of the adipocyte population toward larger hypertrophied cells, reflected in a significant increase in the mean adipocyte size in HFD animals. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
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fig1: Effects of electroacupuncture on body weight, fat accumulation, and adipocyte hypertrophy in high fat diet rats. Animals with high fat diet-induced obesity were treated with EA once by daily for three times (T3) or seven times (T7) in the age of 20 weeks. Pair-feeding was conducted in three separate groups of obese rats to achieve the same body weight during this period; n = 5 rat/group. Flow chart of the study design (a), detailed view of the growth curve during the experimental period, body weight (b, left panel), and food intake was measured on a per-cage basis throughout the study and represents cumulative energy intake (b, right panel) and total adipocyte size expressed at the end of treatment (c). H&E staining showing a decrease in adipocyte size in EA treatment rats, compared with obese animals fed a HFD (e). Distribution of adipocyte sizes indicates a shift in the size of the adipocyte population toward larger hypertrophied cells, reflected in a significant increase in the mean adipocyte size in HFD animals. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.

Mentions: Treatment with T7EA almost abolished the high fat diet-induced epididymal and perirenal fat (Table 1) accumulation in parallel with the body weight reduction. In keeping with this data, serum liver enzymes measured as an indication of liver function followed a similar pattern. Normal chow feeding had no significant effect on liver function in rats. Serum alanine transaminase (ALT) levels were significantly raised in HFD obese animals, suggesting hepatocyte damage. EA treatment reduced ALT levels in HFD rats and tended to reduce aspartate transaminase levels (AST) as well. EA significantly lowered AST and ALT levels, though they remained above normal levels (Table 1). Intense EA treatment of obese rats (HFD + T7 EA) markedly induced weight loss during the first week as compared with obese controls (HFD group). However, the body weight of HFD + T3 EA rats remained higher than that of normal chow controls (Figure 1(b), left panel). Food intake did not significantly differ among the high fat diet group and EA treated groups (Figure 1(b), right panel). After seven times of EA treatment, obese animals had lower body weight; fat pads were also smaller than vehicle control (Figure 1(c)), although both groups consumed the same amount of HFD (Figure 1). EA treatment rats had smaller adipocytes than HFD rats (Figures 1(d) and 1(e)).


Electroacupuncture decreases the leukocyte infiltration to white adipose tissue and attenuates inflammatory response in high fat diet-induced obesity rats.

Wen CK, Lee TY - Evid Based Complement Alternat Med (2014)

Effects of electroacupuncture on body weight, fat accumulation, and adipocyte hypertrophy in high fat diet rats. Animals with high fat diet-induced obesity were treated with EA once by daily for three times (T3) or seven times (T7) in the age of 20 weeks. Pair-feeding was conducted in three separate groups of obese rats to achieve the same body weight during this period; n = 5 rat/group. Flow chart of the study design (a), detailed view of the growth curve during the experimental period, body weight (b, left panel), and food intake was measured on a per-cage basis throughout the study and represents cumulative energy intake (b, right panel) and total adipocyte size expressed at the end of treatment (c). H&E staining showing a decrease in adipocyte size in EA treatment rats, compared with obese animals fed a HFD (e). Distribution of adipocyte sizes indicates a shift in the size of the adipocyte population toward larger hypertrophied cells, reflected in a significant increase in the mean adipocyte size in HFD animals. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig1: Effects of electroacupuncture on body weight, fat accumulation, and adipocyte hypertrophy in high fat diet rats. Animals with high fat diet-induced obesity were treated with EA once by daily for three times (T3) or seven times (T7) in the age of 20 weeks. Pair-feeding was conducted in three separate groups of obese rats to achieve the same body weight during this period; n = 5 rat/group. Flow chart of the study design (a), detailed view of the growth curve during the experimental period, body weight (b, left panel), and food intake was measured on a per-cage basis throughout the study and represents cumulative energy intake (b, right panel) and total adipocyte size expressed at the end of treatment (c). H&E staining showing a decrease in adipocyte size in EA treatment rats, compared with obese animals fed a HFD (e). Distribution of adipocyte sizes indicates a shift in the size of the adipocyte population toward larger hypertrophied cells, reflected in a significant increase in the mean adipocyte size in HFD animals. *P < 0.05, compared with normal chow group; **P < 0.05, compared with HFD animals. NC: normal chow. HFD: high fat diet. EA: electroacupuncture. T3: three times of EA treatment. T7: seven times of EA treatment. Original magnification ×40. Scale bars, 100 μm.
Mentions: Treatment with T7EA almost abolished the high fat diet-induced epididymal and perirenal fat (Table 1) accumulation in parallel with the body weight reduction. In keeping with this data, serum liver enzymes measured as an indication of liver function followed a similar pattern. Normal chow feeding had no significant effect on liver function in rats. Serum alanine transaminase (ALT) levels were significantly raised in HFD obese animals, suggesting hepatocyte damage. EA treatment reduced ALT levels in HFD rats and tended to reduce aspartate transaminase levels (AST) as well. EA significantly lowered AST and ALT levels, though they remained above normal levels (Table 1). Intense EA treatment of obese rats (HFD + T7 EA) markedly induced weight loss during the first week as compared with obese controls (HFD group). However, the body weight of HFD + T3 EA rats remained higher than that of normal chow controls (Figure 1(b), left panel). Food intake did not significantly differ among the high fat diet group and EA treated groups (Figure 1(b), right panel). After seven times of EA treatment, obese animals had lower body weight; fat pads were also smaller than vehicle control (Figure 1(c)), although both groups consumed the same amount of HFD (Figure 1). EA treatment rats had smaller adipocytes than HFD rats (Figures 1(d) and 1(e)).

Bottom Line: Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group.We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD.Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.

ABSTRACT
Suppression of white adipose tissue inflammatory signaling may contribute to the pathogenesis of obesity-induced inflammatory response. However, the precise mechanism of efficacy of acupuncture related to adipose tissue remains poorly understood. In the present study we evaluated the anti-inflammatory activities of 10 Hz electroacupuncture (EA) which was applied at the acupoint Zusanli (ST36) for 20 min per day in high-fat diet- (HFD-) induced obesity model. Treatment lasted for one week. Obese rats treated with EA showed significantly reduced body weight compared with the rats in HFD group. EA decreased the number of F4/80 and CD11b-positive macrophages in epididymal adipose tissue. We found that 10 Hz EA given 7 days/week at ST36 acupoints significantly alleviated macrophage recruitment and then improved the obesity-associated factors of sterol regulatory element-binding protein-1 (SREBP-1) and target genes expression in rats with HFD. Adipose tissue inflammatory responses indicated by tumor necrosis factor-α (TNF-α), IL-6, monocyte chemotactic protein-1 (MCP-1), and CD68 mRNA expression were significantly reduced by EA in obese rats. Additionally, EA was found to significantly reduced serum levels of TNF-α, IL-6, and IL-1 in this model. These results indicated that EA improved adipose tissue inflammatory response in obese rats, at least partly, via attenuation of lipogenesis signaling.

No MeSH data available.


Related in: MedlinePlus