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Inhibition of corneal neovascularization by topical and subconjunctival tigecycline.

Goktas S, Erdogan E, Sakarya R, Sakarya Y, Yılmaz M, Ozcimen M, Unlukal N, Alpfidan I, Tas F, Erdogan E, Bukus A, Ivacık IS - J Ophthalmol (2014)

Bottom Line: Materials and Methods.Control rats received topical (group 2) or subconjunctival (group 4) 0.9% saline.Results.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Konya Training and Research Hospital, Meram, 42090 Konya, Turkey.

ABSTRACT
Objective. To investigate the effects of topical and subconjunctival tigecycline on the prevention of corneal neovascularization. Materials and Methods. Following chemical burn, thirty-two rats were treated daily with topical instillation of 1 mg/mL tigecycline (group 1) or subconjunctival instillation of 1 mg/mL tigecycline (group 3) for 7 days. Control rats received topical (group 2) or subconjunctival (group 4) 0.9% saline. Digital photographs of the cornea were taken on the eighth day after treatment and analyzed to determine the percentage area of the cornea covered by neovascularization. Corneal sections were analyzed histopathologically. Results. The median percentages of corneal neovascularization in groups 1 and 3 were 48% (95% confidence interval (CI), 44.2-55.8%) and 33.5% (95% CI, 26.6-39.2%), respectively. The median percentages of corneal neovascularization of groups 1 and 3 were significantly lower than that of the control group (P = 0.03 and P < 0.001, resp.). Histologic examination of samples from groups 1 and 3 showed lower vascularity than that of control groups. Conclusion. Topical and subconjunctival administration of tigecycline seems to be showing promising therapeutic effects on the prevention of corneal neovascularization. Furthermore, subconjunctival administration of tigecycline is more potent than topical administration in the inhibition of corneal neovascularization.

No MeSH data available.


Related in: MedlinePlus

Histopathologic photographs of cornea after chemical burn. (a) Normal cornea. (b) An example of topically tigecycline-treated eyes revealing less corneal neovascularization. (c) Subconjunctivally tigecycline-treated eyes showing virtually few neovascularization than in control group in stroma. (d) Diffuse and intense neovascularization affecting deep stromal layers after in control eyes.
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fig3: Histopathologic photographs of cornea after chemical burn. (a) Normal cornea. (b) An example of topically tigecycline-treated eyes revealing less corneal neovascularization. (c) Subconjunctivally tigecycline-treated eyes showing virtually few neovascularization than in control group in stroma. (d) Diffuse and intense neovascularization affecting deep stromal layers after in control eyes.

Mentions: Figure 3 illustrates histopathological findings. Maximum density of neovascularization in each group as determined by histopathology is presented in Table 2. Neovascularization intensity in study groups was significantly lower than the control groups with respect to the density of neovascularization. No local or systemic adverse effects were seen from either treatment group.


Inhibition of corneal neovascularization by topical and subconjunctival tigecycline.

Goktas S, Erdogan E, Sakarya R, Sakarya Y, Yılmaz M, Ozcimen M, Unlukal N, Alpfidan I, Tas F, Erdogan E, Bukus A, Ivacık IS - J Ophthalmol (2014)

Histopathologic photographs of cornea after chemical burn. (a) Normal cornea. (b) An example of topically tigecycline-treated eyes revealing less corneal neovascularization. (c) Subconjunctivally tigecycline-treated eyes showing virtually few neovascularization than in control group in stroma. (d) Diffuse and intense neovascularization affecting deep stromal layers after in control eyes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150514&req=5

fig3: Histopathologic photographs of cornea after chemical burn. (a) Normal cornea. (b) An example of topically tigecycline-treated eyes revealing less corneal neovascularization. (c) Subconjunctivally tigecycline-treated eyes showing virtually few neovascularization than in control group in stroma. (d) Diffuse and intense neovascularization affecting deep stromal layers after in control eyes.
Mentions: Figure 3 illustrates histopathological findings. Maximum density of neovascularization in each group as determined by histopathology is presented in Table 2. Neovascularization intensity in study groups was significantly lower than the control groups with respect to the density of neovascularization. No local or systemic adverse effects were seen from either treatment group.

Bottom Line: Materials and Methods.Control rats received topical (group 2) or subconjunctival (group 4) 0.9% saline.Results.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Konya Training and Research Hospital, Meram, 42090 Konya, Turkey.

ABSTRACT
Objective. To investigate the effects of topical and subconjunctival tigecycline on the prevention of corneal neovascularization. Materials and Methods. Following chemical burn, thirty-two rats were treated daily with topical instillation of 1 mg/mL tigecycline (group 1) or subconjunctival instillation of 1 mg/mL tigecycline (group 3) for 7 days. Control rats received topical (group 2) or subconjunctival (group 4) 0.9% saline. Digital photographs of the cornea were taken on the eighth day after treatment and analyzed to determine the percentage area of the cornea covered by neovascularization. Corneal sections were analyzed histopathologically. Results. The median percentages of corneal neovascularization in groups 1 and 3 were 48% (95% confidence interval (CI), 44.2-55.8%) and 33.5% (95% CI, 26.6-39.2%), respectively. The median percentages of corneal neovascularization of groups 1 and 3 were significantly lower than that of the control group (P = 0.03 and P < 0.001, resp.). Histologic examination of samples from groups 1 and 3 showed lower vascularity than that of control groups. Conclusion. Topical and subconjunctival administration of tigecycline seems to be showing promising therapeutic effects on the prevention of corneal neovascularization. Furthermore, subconjunctival administration of tigecycline is more potent than topical administration in the inhibition of corneal neovascularization.

No MeSH data available.


Related in: MedlinePlus