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Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

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Related in: MedlinePlus

Apoptosis induction by jaboticaba seed water extract treatment of HSC-3 cells. (a) HSC-3 cells treated with jaboticaba seed water extract for 24 h at desired concentrations were stained with Annexin V-FITC and propidium iodide (PI). The Annexin V-FITC signal is shown on the x-axis and PI signal is shown on the y-axis. The apoptotic populations of cells were significantly increased in treatment groups in a dose-dependent manner. (b) Flow cytometric analysis was performed after a 48 h treatment to determine caspase-3 activity, which was found to be activated in a dose-dependent manner.
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fig3: Apoptosis induction by jaboticaba seed water extract treatment of HSC-3 cells. (a) HSC-3 cells treated with jaboticaba seed water extract for 24 h at desired concentrations were stained with Annexin V-FITC and propidium iodide (PI). The Annexin V-FITC signal is shown on the x-axis and PI signal is shown on the y-axis. The apoptotic populations of cells were significantly increased in treatment groups in a dose-dependent manner. (b) Flow cytometric analysis was performed after a 48 h treatment to determine caspase-3 activity, which was found to be activated in a dose-dependent manner.

Mentions: To further understand whether the decrease in cell numbers observed with the MTT assay was due to the slowdown of the cell cycle or the increase of apoptosis, we examined cell behavior after treatment using Annexin V/PI staining. Figure 3(a) shows the Annexin V (+) population change after adding jaboticaba seed/water extract to HSC-3 cells. In the control group, the Annexin V (+) population was less than 5%. When HSC-3 cells were treated with the extract, the population increased to 15.2% and 57.1% in 10 and 50 μg/mL treatments. In addition, the induction of caspase-3 activity was also examined by flow cytometry (Figure 3(b)). The increasing level of active caspase-3 with the elevated concentration of the extract showed that the apoptosis of HSC-3 cells was induced by the jaboticaba seed water extract.


Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Apoptosis induction by jaboticaba seed water extract treatment of HSC-3 cells. (a) HSC-3 cells treated with jaboticaba seed water extract for 24 h at desired concentrations were stained with Annexin V-FITC and propidium iodide (PI). The Annexin V-FITC signal is shown on the x-axis and PI signal is shown on the y-axis. The apoptotic populations of cells were significantly increased in treatment groups in a dose-dependent manner. (b) Flow cytometric analysis was performed after a 48 h treatment to determine caspase-3 activity, which was found to be activated in a dose-dependent manner.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150497&req=5

fig3: Apoptosis induction by jaboticaba seed water extract treatment of HSC-3 cells. (a) HSC-3 cells treated with jaboticaba seed water extract for 24 h at desired concentrations were stained with Annexin V-FITC and propidium iodide (PI). The Annexin V-FITC signal is shown on the x-axis and PI signal is shown on the y-axis. The apoptotic populations of cells were significantly increased in treatment groups in a dose-dependent manner. (b) Flow cytometric analysis was performed after a 48 h treatment to determine caspase-3 activity, which was found to be activated in a dose-dependent manner.
Mentions: To further understand whether the decrease in cell numbers observed with the MTT assay was due to the slowdown of the cell cycle or the increase of apoptosis, we examined cell behavior after treatment using Annexin V/PI staining. Figure 3(a) shows the Annexin V (+) population change after adding jaboticaba seed/water extract to HSC-3 cells. In the control group, the Annexin V (+) population was less than 5%. When HSC-3 cells were treated with the extract, the population increased to 15.2% and 57.1% in 10 and 50 μg/mL treatments. In addition, the induction of caspase-3 activity was also examined by flow cytometry (Figure 3(b)). The increasing level of active caspase-3 with the elevated concentration of the extract showed that the apoptosis of HSC-3 cells was induced by the jaboticaba seed water extract.

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

Show MeSH
Related in: MedlinePlus