Limits...
Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

Show MeSH

Related in: MedlinePlus

Water extract of jaboticaba seeds possessed the most potent cytotoxic activity. Cell survival rate of HSC-3 cells after 24 h treatment with various extracts showed that the water extract of jaboticaba seeds had the most significant cytotoxic activity compared to extracts from other portions of the plant. The IC50 of water extract of jaboticaba seeds in HSC-3 cells was approximately 15 μg/mL. Each point represents the mean and SD of six determinations.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150497&req=5

fig2: Water extract of jaboticaba seeds possessed the most potent cytotoxic activity. Cell survival rate of HSC-3 cells after 24 h treatment with various extracts showed that the water extract of jaboticaba seeds had the most significant cytotoxic activity compared to extracts from other portions of the plant. The IC50 of water extract of jaboticaba seeds in HSC-3 cells was approximately 15 μg/mL. Each point represents the mean and SD of six determinations.

Mentions: To evaluate which part of jaboticaba possesses the most potent antiproliferating effect on oral cancer cells, we treated HSC-3 cells with jaboticaba water or ethanol extracts from different parts of the plant. Cytotoxicity on HSC-3 cells determined using the MTT assay is shown in Figure 2. Of the four types of extracts (jaboticaba seed/water, seed/ethanol, stem/ethanol, and peel/ethanol), only seed/water showed a marked inhibition effect on HSC-3 cell viability and it was in a dose-dependent manner. After a 24 hr treatment, the IC50 of jaboticaba seed/water extract was approximately 15 μg/mL. These data suggest that the seed/water extract of jaboticaba is the most potent of all of the extracts we examined.


Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Water extract of jaboticaba seeds possessed the most potent cytotoxic activity. Cell survival rate of HSC-3 cells after 24 h treatment with various extracts showed that the water extract of jaboticaba seeds had the most significant cytotoxic activity compared to extracts from other portions of the plant. The IC50 of water extract of jaboticaba seeds in HSC-3 cells was approximately 15 μg/mL. Each point represents the mean and SD of six determinations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150497&req=5

fig2: Water extract of jaboticaba seeds possessed the most potent cytotoxic activity. Cell survival rate of HSC-3 cells after 24 h treatment with various extracts showed that the water extract of jaboticaba seeds had the most significant cytotoxic activity compared to extracts from other portions of the plant. The IC50 of water extract of jaboticaba seeds in HSC-3 cells was approximately 15 μg/mL. Each point represents the mean and SD of six determinations.
Mentions: To evaluate which part of jaboticaba possesses the most potent antiproliferating effect on oral cancer cells, we treated HSC-3 cells with jaboticaba water or ethanol extracts from different parts of the plant. Cytotoxicity on HSC-3 cells determined using the MTT assay is shown in Figure 2. Of the four types of extracts (jaboticaba seed/water, seed/ethanol, stem/ethanol, and peel/ethanol), only seed/water showed a marked inhibition effect on HSC-3 cell viability and it was in a dose-dependent manner. After a 24 hr treatment, the IC50 of jaboticaba seed/water extract was approximately 15 μg/mL. These data suggest that the seed/water extract of jaboticaba is the most potent of all of the extracts we examined.

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

Show MeSH
Related in: MedlinePlus