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Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

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Antioxidative activity of different parts of jaboticaba.Using DPPH and ABTS assays, the water extract of jaboticaba seeds was found to have the best scavenging activity compared to other portions of the plant. Trolox served as a positive control.
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fig1: Antioxidative activity of different parts of jaboticaba.Using DPPH and ABTS assays, the water extract of jaboticaba seeds was found to have the best scavenging activity compared to other portions of the plant. Trolox served as a positive control.

Mentions: The results of the determination of antioxidant activity of different extracts using two methods (DPPH and ABTS) are presented in Figure 1. For DPPH scavenging activity the water extract of jaboticaba seed was more active than the ethanol extract of seed and other parts of jaboticaba. The SC50 of ethanol extracts of peel, stem, seeds, and water extract of seeds were 0.049, 0.017, 0.027, and 0.0059, respectively. The SC50 of water extract of seed was close to the positive control, Trolox (0.0053).


Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.

Wang WH, Tyan YC, Chen ZS, Lin CG, Yang MH, Yuan SS, Tsai WC - Biomed Res Int (2014)

Antioxidative activity of different parts of jaboticaba.Using DPPH and ABTS assays, the water extract of jaboticaba seeds was found to have the best scavenging activity compared to other portions of the plant. Trolox served as a positive control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150497&req=5

fig1: Antioxidative activity of different parts of jaboticaba.Using DPPH and ABTS assays, the water extract of jaboticaba seeds was found to have the best scavenging activity compared to other portions of the plant. Trolox served as a positive control.
Mentions: The results of the determination of antioxidant activity of different extracts using two methods (DPPH and ABTS) are presented in Figure 1. For DPPH scavenging activity the water extract of jaboticaba seed was more active than the ethanol extract of seed and other parts of jaboticaba. The SC50 of ethanol extracts of peel, stem, seeds, and water extract of seeds were 0.049, 0.017, 0.027, and 0.0059, respectively. The SC50 of water extract of seed was close to the positive control, Trolox (0.0053).

Bottom Line: Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects.The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined.The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.

ABSTRACT
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.

Show MeSH
Related in: MedlinePlus