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Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.

Kong Y, Wei C, Hou Z, Wang Z, Yuan J, Yu J, Zhao Y, Tang Y, Gao M - J Anal Methods Chem (2014)

Bottom Line: The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates.Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L.Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved.

View Article: PubMed Central - PubMed

Affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

ABSTRACT
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.

No MeSH data available.


Influences of injection volume on migration times and Rs, effects of voltages on stacking.
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fig4: Influences of injection volume on migration times and Rs, effects of voltages on stacking.

Mentions: As the injection time (presented as percent of the efficient length) was closely related to sensitivity of the method, the maximum injection time was carefully selected in this section. Figure 4 showed that migration times increased (as a result of decreasing EOF) while the Rs values decreased (mainly caused by the decreasing efficient capillary length) with increasing injection times (similar to the results of Gao's work [25]). Based on the Rs value between MEL and interferent, 20% of the efficient capillary length (50 mBar × 40.0 s) was considered as a best injection percent (Rs = 1.75).


Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.

Kong Y, Wei C, Hou Z, Wang Z, Yuan J, Yu J, Zhao Y, Tang Y, Gao M - J Anal Methods Chem (2014)

Influences of injection volume on migration times and Rs, effects of voltages on stacking.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150491&req=5

fig4: Influences of injection volume on migration times and Rs, effects of voltages on stacking.
Mentions: As the injection time (presented as percent of the efficient length) was closely related to sensitivity of the method, the maximum injection time was carefully selected in this section. Figure 4 showed that migration times increased (as a result of decreasing EOF) while the Rs values decreased (mainly caused by the decreasing efficient capillary length) with increasing injection times (similar to the results of Gao's work [25]). Based on the Rs value between MEL and interferent, 20% of the efficient capillary length (50 mBar × 40.0 s) was considered as a best injection percent (Rs = 1.75).

Bottom Line: The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates.Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L.Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved.

View Article: PubMed Central - PubMed

Affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

ABSTRACT
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.

No MeSH data available.