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Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.

Kong Y, Wei C, Hou Z, Wang Z, Yuan J, Yu J, Zhao Y, Tang Y, Gao M - J Anal Methods Chem (2014)

Bottom Line: The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates.Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L.Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved.

View Article: PubMed Central - PubMed

Affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

ABSTRACT
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.

No MeSH data available.


Related in: MedlinePlus

Electropherograms of milk treated with different percentages of acetonitrile. Condition: samples were treated with different percentages of acetonitrile (v/v) and were injected hydrodynamically at 50 mBar for 50 s. All samples contain 10 mmol/L NaCl and 8 mmol/L pH 2.8 phosphate solution and 3 μmol/L MEL.
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fig3: Electropherograms of milk treated with different percentages of acetonitrile. Condition: samples were treated with different percentages of acetonitrile (v/v) and were injected hydrodynamically at 50 mBar for 50 s. All samples contain 10 mmol/L NaCl and 8 mmol/L pH 2.8 phosphate solution and 3 μmol/L MEL.

Mentions: The effect of the acetonitrile concentration in sample on stacking was studied and optimized as organic reagent acted as accelerator for speeding up the velocities of analytes [15]. It could be seen (Figure 3), as the percent of the acetonitrile was lower than 50%, that the baseline around MEL became unsatisfied (arrow pointed in Figure 3) for separation and quantification, although the Rs value was higher enough between the interferent and MEL (Rs > 3.5). When the percentage was larger than 50%, baseline separation and stacking were both achieved. Meanwhile, the Rs decreased from 3.27 (60%) to 1.53 (80%) as the percentage of acetonitrile increased. Therefore, a percentage of 60% was considered as the better condition that provided less sample dilution and better Rs.


Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.

Kong Y, Wei C, Hou Z, Wang Z, Yuan J, Yu J, Zhao Y, Tang Y, Gao M - J Anal Methods Chem (2014)

Electropherograms of milk treated with different percentages of acetonitrile. Condition: samples were treated with different percentages of acetonitrile (v/v) and were injected hydrodynamically at 50 mBar for 50 s. All samples contain 10 mmol/L NaCl and 8 mmol/L pH 2.8 phosphate solution and 3 μmol/L MEL.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150491&req=5

fig3: Electropherograms of milk treated with different percentages of acetonitrile. Condition: samples were treated with different percentages of acetonitrile (v/v) and were injected hydrodynamically at 50 mBar for 50 s. All samples contain 10 mmol/L NaCl and 8 mmol/L pH 2.8 phosphate solution and 3 μmol/L MEL.
Mentions: The effect of the acetonitrile concentration in sample on stacking was studied and optimized as organic reagent acted as accelerator for speeding up the velocities of analytes [15]. It could be seen (Figure 3), as the percent of the acetonitrile was lower than 50%, that the baseline around MEL became unsatisfied (arrow pointed in Figure 3) for separation and quantification, although the Rs value was higher enough between the interferent and MEL (Rs > 3.5). When the percentage was larger than 50%, baseline separation and stacking were both achieved. Meanwhile, the Rs decreased from 3.27 (60%) to 1.53 (80%) as the percentage of acetonitrile increased. Therefore, a percentage of 60% was considered as the better condition that provided less sample dilution and better Rs.

Bottom Line: The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates.Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L.Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved.

View Article: PubMed Central - PubMed

Affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

ABSTRACT
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.

No MeSH data available.


Related in: MedlinePlus