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Liquid phase separation of proteins based on electrophoretic effects in an electrospray setup during sample introduction into a gas-phase electrophoretic mobility molecular analyzer (CE-GEMMA/CE-ES-DMA).

Weiss VU, Kerul L, Kallinger P, Szymanski WW, Marchetti-Deschmann M, Allmaier G - Anal. Chim. Acta (2014)

Bottom Line: This makes the EM determination of individual species sometimes difficult, if not impossible.This finding was consecutively applied for on-line desalting allowing EM diameter determination of analytes despite a high salt concentration within samples.Results demonstrate the proof of concept of such an approach and additionally illustrate the high potential of a future on-line coupling of a capillary electrophoresis to a GEMMA instrument.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.

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Related in: MedlinePlus

Analyte migration through the capillary in CM: BSA (black) and IgG (grey) samples were investigated under conditions as given in Fig. 3. Again, differences in analyte migration are detectable.
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fig0020: Analyte migration through the capillary in CM: BSA (black) and IgG (grey) samples were investigated under conditions as given in Fig. 3. Again, differences in analyte migration are detectable.

Mentions: The measurements of BSA and IgG solutions in ammonium acetate in CM were taken to confirm the results described already for FM (one protein per respective sample). The corresponding signals of analytes after injection to the GEMMA instrument are shown in Fig. 4 (n = 3 measurements). The difference between the migration time of IgG (grey) and BSA (black) is about 20 s and the peak width for both analytes about 30 s. These results again demonstrate the CE separation potential within the nano ES capillary of the commercial GEMMA instrument.


Liquid phase separation of proteins based on electrophoretic effects in an electrospray setup during sample introduction into a gas-phase electrophoretic mobility molecular analyzer (CE-GEMMA/CE-ES-DMA).

Weiss VU, Kerul L, Kallinger P, Szymanski WW, Marchetti-Deschmann M, Allmaier G - Anal. Chim. Acta (2014)

Analyte migration through the capillary in CM: BSA (black) and IgG (grey) samples were investigated under conditions as given in Fig. 3. Again, differences in analyte migration are detectable.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150488&req=5

fig0020: Analyte migration through the capillary in CM: BSA (black) and IgG (grey) samples were investigated under conditions as given in Fig. 3. Again, differences in analyte migration are detectable.
Mentions: The measurements of BSA and IgG solutions in ammonium acetate in CM were taken to confirm the results described already for FM (one protein per respective sample). The corresponding signals of analytes after injection to the GEMMA instrument are shown in Fig. 4 (n = 3 measurements). The difference between the migration time of IgG (grey) and BSA (black) is about 20 s and the peak width for both analytes about 30 s. These results again demonstrate the CE separation potential within the nano ES capillary of the commercial GEMMA instrument.

Bottom Line: This makes the EM determination of individual species sometimes difficult, if not impossible.This finding was consecutively applied for on-line desalting allowing EM diameter determination of analytes despite a high salt concentration within samples.Results demonstrate the proof of concept of such an approach and additionally illustrate the high potential of a future on-line coupling of a capillary electrophoresis to a GEMMA instrument.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.

Show MeSH
Related in: MedlinePlus