Liquid phase separation of proteins based on electrophoretic effects in an electrospray setup during sample introduction into a gas-phase electrophoretic mobility molecular analyzer (CE-GEMMA/CE-ES-DMA).
Bottom Line: This makes the EM determination of individual species sometimes difficult, if not impossible.This finding was consecutively applied for on-line desalting allowing EM diameter determination of analytes despite a high salt concentration within samples.Results demonstrate the proof of concept of such an approach and additionally illustrate the high potential of a future on-line coupling of a capillary electrophoresis to a GEMMA instrument.
Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.Show MeSH
Related in: MedlinePlus
Mentions: Calculated results for Δppsid are shown in Fig. 2(A); upon reduction of the applied pressure to the sample chamber, the time needed for analytes to pass through the nano ES capillary of the instrument is increased significantly.
Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.