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Liquid phase separation of proteins based on electrophoretic effects in an electrospray setup during sample introduction into a gas-phase electrophoretic mobility molecular analyzer (CE-GEMMA/CE-ES-DMA).

Weiss VU, Kerul L, Kallinger P, Szymanski WW, Marchetti-Deschmann M, Allmaier G - Anal. Chim. Acta (2014)

Bottom Line: This makes the EM determination of individual species sometimes difficult, if not impossible.This finding was consecutively applied for on-line desalting allowing EM diameter determination of analytes despite a high salt concentration within samples.Results demonstrate the proof of concept of such an approach and additionally illustrate the high potential of a future on-line coupling of a capillary electrophoresis to a GEMMA instrument.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.

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Related in: MedlinePlus

Dependence of analyte migration time through the nano ES capillary on the applied pressure to the sample chamber (A): the migration time of analytes was calculated by Hagen–Poiseuille equation. Electrophoretic net mobilities of analytes under comparable conditions as during the nano ES process (B): the EOF mobility is increasing with increasing pH values, whereas the electrophoretic net mobilities of analytes do not change significantly.
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fig0010: Dependence of analyte migration time through the nano ES capillary on the applied pressure to the sample chamber (A): the migration time of analytes was calculated by Hagen–Poiseuille equation. Electrophoretic net mobilities of analytes under comparable conditions as during the nano ES process (B): the EOF mobility is increasing with increasing pH values, whereas the electrophoretic net mobilities of analytes do not change significantly.

Mentions: Calculated results for Δppsid are shown in Fig. 2(A); upon reduction of the applied pressure to the sample chamber, the time needed for analytes to pass through the nano ES capillary of the instrument is increased significantly.


Liquid phase separation of proteins based on electrophoretic effects in an electrospray setup during sample introduction into a gas-phase electrophoretic mobility molecular analyzer (CE-GEMMA/CE-ES-DMA).

Weiss VU, Kerul L, Kallinger P, Szymanski WW, Marchetti-Deschmann M, Allmaier G - Anal. Chim. Acta (2014)

Dependence of analyte migration time through the nano ES capillary on the applied pressure to the sample chamber (A): the migration time of analytes was calculated by Hagen–Poiseuille equation. Electrophoretic net mobilities of analytes under comparable conditions as during the nano ES process (B): the EOF mobility is increasing with increasing pH values, whereas the electrophoretic net mobilities of analytes do not change significantly.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150488&req=5

fig0010: Dependence of analyte migration time through the nano ES capillary on the applied pressure to the sample chamber (A): the migration time of analytes was calculated by Hagen–Poiseuille equation. Electrophoretic net mobilities of analytes under comparable conditions as during the nano ES process (B): the EOF mobility is increasing with increasing pH values, whereas the electrophoretic net mobilities of analytes do not change significantly.
Mentions: Calculated results for Δppsid are shown in Fig. 2(A); upon reduction of the applied pressure to the sample chamber, the time needed for analytes to pass through the nano ES capillary of the instrument is increased significantly.

Bottom Line: This makes the EM determination of individual species sometimes difficult, if not impossible.This finding was consecutively applied for on-line desalting allowing EM diameter determination of analytes despite a high salt concentration within samples.Results demonstrate the proof of concept of such an approach and additionally illustrate the high potential of a future on-line coupling of a capillary electrophoresis to a GEMMA instrument.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.

Show MeSH
Related in: MedlinePlus