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CC chemokine ligand 18 correlates with malignant progression of prostate cancer.

Chen G, Liang YX, Zhu JG, Fu X, Chen YF, Mo RJ, Zhou L, Fu H, Bi XC, He HC, Yang SB, Wu YD, Jiang FN, Zhong WD - Biomed Res Int (2014)

Bottom Line: However, its involvement in human prostate cancer has not been fully elucidated.The effects of PCa cell migration, invasion, and apoptosis were tested.CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.

ABSTRACT

Background and aim: CC chemokine ligand 18 (CCL18) promotes malignant behaviors of various human cancer types. However, its involvement in human prostate cancer has not been fully elucidated. The aim of this study was to investigate the role of CCL18 in PCa.

Methods: Expression of CCL18 at mRNA and protein levels was detected using real-time qRT-PCR and immunohistochemistry analysis. We analyzed the associations of CCL18 expression with clinical features of human PCa. The effects of PCa cell migration, invasion, and apoptosis were tested. The efficiency of CCL18 on prostate tumor growth was assessed in a subcutaneous xenograft model.

Results: CCL18 expression was upregulated (both P < 0.01) in PCa tissues compared with those in noncancerous prostate tissues. CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate. Furthermore, subcutaneous homografts models showed the increased tumor growth and tumor vascularization with the CCL18 stimulation, and the expression of Ki67, PCNA, and CD31 in CCL18 stimulation mice was also significantly increased.

Conclusions: Our data offer the convincing evidence that the upregulation of CCL18 may be involved in the malignant progression of PCa.

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Expression pattern and localization of CCL18 protein in prostate cancer (PCa) and adjacent benign prostate tissues detected by immunohistochemistry analysis. ((a)–(f)) Immunostainings for CCL18 protein in PCa and adjacent benign prostate tissues. (g) Immunoreactivity score (IRS) of CCL18 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001). ((h)–(m)) Immunostainings for CD68 protein in PCa and adjacent benign prostate tissues. (n) IRS of CD68 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001).
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fig2: Expression pattern and localization of CCL18 protein in prostate cancer (PCa) and adjacent benign prostate tissues detected by immunohistochemistry analysis. ((a)–(f)) Immunostainings for CCL18 protein in PCa and adjacent benign prostate tissues. (g) Immunoreactivity score (IRS) of CCL18 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001). ((h)–(m)) Immunostainings for CD68 protein in PCa and adjacent benign prostate tissues. (n) IRS of CD68 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001).

Mentions: In addition, the expression patterns and localizations of CCL18 in 80 PCa tissues and 95 adjacent benign prostate tissues were examined using immunohistochemical analysis. Please see detailed information on immunostainings of our TMA samples in supplementary files Supplementary Figure S1 and Table S1 in the Supplementary Material available online at http://dx.doi.org/10.1155/2014/230183. Representative pictures of immunostainings of CCL18 protein are shown in Figures 2(a)~2(f). In benign prostate tissues, CCL18 immunostainings occurred mainly in the cytoplasm of macrophages and the surrounding gland epithelial cells. In PCa tissues, positive immunostainings of CCL18 were observed in the cytoplasm of macrophages and cancer cells. In line with the results of western blot analysis, the IRS of CCL18 protein in PCa tissues was also significantly higher than those in benign prostate tissues (CCL18: PCa = 4.09 ± 1.81 versus benign = 2.31 ± 2.11, P < 0.01, Figure 2(g)). Since macrophage may secrete CCL18 protein which may be correlated with PCa, we also detected the expression of CD68, a marker for macrophage, in these tissue sections. As a result shown in Figures 2(h)~2(n), the expression of CD68 in PCa tissues was also higher than those in benign prostate tissues (CD68: PCa = 2.60 ± 1.30 versus benign = 1.26 ± 0.71, P < 0.01). More interestingly, the expression levels of CD68 in PCa tissues were significantly correlated with those of CCL18 (R = 0.337, P < 0.01).


CC chemokine ligand 18 correlates with malignant progression of prostate cancer.

Chen G, Liang YX, Zhu JG, Fu X, Chen YF, Mo RJ, Zhou L, Fu H, Bi XC, He HC, Yang SB, Wu YD, Jiang FN, Zhong WD - Biomed Res Int (2014)

Expression pattern and localization of CCL18 protein in prostate cancer (PCa) and adjacent benign prostate tissues detected by immunohistochemistry analysis. ((a)–(f)) Immunostainings for CCL18 protein in PCa and adjacent benign prostate tissues. (g) Immunoreactivity score (IRS) of CCL18 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001). ((h)–(m)) Immunostainings for CD68 protein in PCa and adjacent benign prostate tissues. (n) IRS of CD68 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
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fig2: Expression pattern and localization of CCL18 protein in prostate cancer (PCa) and adjacent benign prostate tissues detected by immunohistochemistry analysis. ((a)–(f)) Immunostainings for CCL18 protein in PCa and adjacent benign prostate tissues. (g) Immunoreactivity score (IRS) of CCL18 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001). ((h)–(m)) Immunostainings for CD68 protein in PCa and adjacent benign prostate tissues. (n) IRS of CD68 protein in PCa tissues was higher than that in adjacent benign tissues (P < 0.001).
Mentions: In addition, the expression patterns and localizations of CCL18 in 80 PCa tissues and 95 adjacent benign prostate tissues were examined using immunohistochemical analysis. Please see detailed information on immunostainings of our TMA samples in supplementary files Supplementary Figure S1 and Table S1 in the Supplementary Material available online at http://dx.doi.org/10.1155/2014/230183. Representative pictures of immunostainings of CCL18 protein are shown in Figures 2(a)~2(f). In benign prostate tissues, CCL18 immunostainings occurred mainly in the cytoplasm of macrophages and the surrounding gland epithelial cells. In PCa tissues, positive immunostainings of CCL18 were observed in the cytoplasm of macrophages and cancer cells. In line with the results of western blot analysis, the IRS of CCL18 protein in PCa tissues was also significantly higher than those in benign prostate tissues (CCL18: PCa = 4.09 ± 1.81 versus benign = 2.31 ± 2.11, P < 0.01, Figure 2(g)). Since macrophage may secrete CCL18 protein which may be correlated with PCa, we also detected the expression of CD68, a marker for macrophage, in these tissue sections. As a result shown in Figures 2(h)~2(n), the expression of CD68 in PCa tissues was also higher than those in benign prostate tissues (CD68: PCa = 2.60 ± 1.30 versus benign = 1.26 ± 0.71, P < 0.01). More interestingly, the expression levels of CD68 in PCa tissues were significantly correlated with those of CCL18 (R = 0.337, P < 0.01).

Bottom Line: However, its involvement in human prostate cancer has not been fully elucidated.The effects of PCa cell migration, invasion, and apoptosis were tested.CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.

ABSTRACT

Background and aim: CC chemokine ligand 18 (CCL18) promotes malignant behaviors of various human cancer types. However, its involvement in human prostate cancer has not been fully elucidated. The aim of this study was to investigate the role of CCL18 in PCa.

Methods: Expression of CCL18 at mRNA and protein levels was detected using real-time qRT-PCR and immunohistochemistry analysis. We analyzed the associations of CCL18 expression with clinical features of human PCa. The effects of PCa cell migration, invasion, and apoptosis were tested. The efficiency of CCL18 on prostate tumor growth was assessed in a subcutaneous xenograft model.

Results: CCL18 expression was upregulated (both P < 0.01) in PCa tissues compared with those in noncancerous prostate tissues. CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate. Furthermore, subcutaneous homografts models showed the increased tumor growth and tumor vascularization with the CCL18 stimulation, and the expression of Ki67, PCNA, and CD31 in CCL18 stimulation mice was also significantly increased.

Conclusions: Our data offer the convincing evidence that the upregulation of CCL18 may be involved in the malignant progression of PCa.

Show MeSH
Related in: MedlinePlus