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CC chemokine ligand 18 correlates with malignant progression of prostate cancer.

Chen G, Liang YX, Zhu JG, Fu X, Chen YF, Mo RJ, Zhou L, Fu H, Bi XC, He HC, Yang SB, Wu YD, Jiang FN, Zhong WD - Biomed Res Int (2014)

Bottom Line: However, its involvement in human prostate cancer has not been fully elucidated.The effects of PCa cell migration, invasion, and apoptosis were tested.CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.

ABSTRACT

Background and aim: CC chemokine ligand 18 (CCL18) promotes malignant behaviors of various human cancer types. However, its involvement in human prostate cancer has not been fully elucidated. The aim of this study was to investigate the role of CCL18 in PCa.

Methods: Expression of CCL18 at mRNA and protein levels was detected using real-time qRT-PCR and immunohistochemistry analysis. We analyzed the associations of CCL18 expression with clinical features of human PCa. The effects of PCa cell migration, invasion, and apoptosis were tested. The efficiency of CCL18 on prostate tumor growth was assessed in a subcutaneous xenograft model.

Results: CCL18 expression was upregulated (both P < 0.01) in PCa tissues compared with those in noncancerous prostate tissues. CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate. Furthermore, subcutaneous homografts models showed the increased tumor growth and tumor vascularization with the CCL18 stimulation, and the expression of Ki67, PCNA, and CD31 in CCL18 stimulation mice was also significantly increased.

Conclusions: Our data offer the convincing evidence that the upregulation of CCL18 may be involved in the malignant progression of PCa.

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Related in: MedlinePlus

CCL18 mRNA and protein expression in prostate cancer (PCa) and adjacent benign prostate tissues detected by real-time quantitative RT-PCR assay (b) and western blot analysis ((a) and (c)). The statistical analysis showed that CCL18 mRNA average expression level (n = 10) was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (n = 10, P < 0.001, (b)), which was consistent with the findings of western blot analysis on CCL18 protein expression (n = 10, PCa: 1.12 ± 0.24 versus benign: 0.26 ± 0.16, P = 0.017, (a) and (c)).
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fig1: CCL18 mRNA and protein expression in prostate cancer (PCa) and adjacent benign prostate tissues detected by real-time quantitative RT-PCR assay (b) and western blot analysis ((a) and (c)). The statistical analysis showed that CCL18 mRNA average expression level (n = 10) was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (n = 10, P < 0.001, (b)), which was consistent with the findings of western blot analysis on CCL18 protein expression (n = 10, PCa: 1.12 ± 0.24 versus benign: 0.26 ± 0.16, P = 0.017, (a) and (c)).

Mentions: qRT-PCR and western blot analyses were performed to detect the mRNA and protein expression levels of CCL18 in 10 PCa tissues and 10 adjacent benign prostate tissues as shown in Figure 1. Relative expression ratio was defined as the expression levels of CCL18 mRNA to those of the internal reference gene, β-actin mRNA. The statistical analysis showed that CCL18 mRNA expression level was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (P < 0.01, Figure 1(a)), which was consistent with the findings of western blot analysis on CCL18 protein expression (PCa: 1.06 ± 0.23 versus benign: 0.32 ± 0.24, P < 0.01, Figures 1(b) and 1(c)).


CC chemokine ligand 18 correlates with malignant progression of prostate cancer.

Chen G, Liang YX, Zhu JG, Fu X, Chen YF, Mo RJ, Zhou L, Fu H, Bi XC, He HC, Yang SB, Wu YD, Jiang FN, Zhong WD - Biomed Res Int (2014)

CCL18 mRNA and protein expression in prostate cancer (PCa) and adjacent benign prostate tissues detected by real-time quantitative RT-PCR assay (b) and western blot analysis ((a) and (c)). The statistical analysis showed that CCL18 mRNA average expression level (n = 10) was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (n = 10, P < 0.001, (b)), which was consistent with the findings of western blot analysis on CCL18 protein expression (n = 10, PCa: 1.12 ± 0.24 versus benign: 0.26 ± 0.16, P = 0.017, (a) and (c)).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4150478&req=5

fig1: CCL18 mRNA and protein expression in prostate cancer (PCa) and adjacent benign prostate tissues detected by real-time quantitative RT-PCR assay (b) and western blot analysis ((a) and (c)). The statistical analysis showed that CCL18 mRNA average expression level (n = 10) was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (n = 10, P < 0.001, (b)), which was consistent with the findings of western blot analysis on CCL18 protein expression (n = 10, PCa: 1.12 ± 0.24 versus benign: 0.26 ± 0.16, P = 0.017, (a) and (c)).
Mentions: qRT-PCR and western blot analyses were performed to detect the mRNA and protein expression levels of CCL18 in 10 PCa tissues and 10 adjacent benign prostate tissues as shown in Figure 1. Relative expression ratio was defined as the expression levels of CCL18 mRNA to those of the internal reference gene, β-actin mRNA. The statistical analysis showed that CCL18 mRNA expression level was significantly upregulated in PCa tissues compared with those in noncancerous prostate tissues (P < 0.01, Figure 1(a)), which was consistent with the findings of western blot analysis on CCL18 protein expression (PCa: 1.06 ± 0.23 versus benign: 0.32 ± 0.24, P < 0.01, Figures 1(b) and 1(c)).

Bottom Line: However, its involvement in human prostate cancer has not been fully elucidated.The effects of PCa cell migration, invasion, and apoptosis were tested.CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.

ABSTRACT

Background and aim: CC chemokine ligand 18 (CCL18) promotes malignant behaviors of various human cancer types. However, its involvement in human prostate cancer has not been fully elucidated. The aim of this study was to investigate the role of CCL18 in PCa.

Methods: Expression of CCL18 at mRNA and protein levels was detected using real-time qRT-PCR and immunohistochemistry analysis. We analyzed the associations of CCL18 expression with clinical features of human PCa. The effects of PCa cell migration, invasion, and apoptosis were tested. The efficiency of CCL18 on prostate tumor growth was assessed in a subcutaneous xenograft model.

Results: CCL18 expression was upregulated (both P < 0.01) in PCa tissues compared with those in noncancerous prostate tissues. CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate. Furthermore, subcutaneous homografts models showed the increased tumor growth and tumor vascularization with the CCL18 stimulation, and the expression of Ki67, PCNA, and CD31 in CCL18 stimulation mice was also significantly increased.

Conclusions: Our data offer the convincing evidence that the upregulation of CCL18 may be involved in the malignant progression of PCa.

Show MeSH
Related in: MedlinePlus