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Oxidative stress and NO signalling in the root apex as an early response to changes in gravity conditions.

Mugnai S, Pandolfi C, Masi E, Azzarello E, Monetti E, Comparini D, Voigt B, Volkmann D, Mancuso S - Biomed Res Int (2014)

Bottom Line: The same results were obtained by ROS measurement.The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated.Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

View Article: PubMed Central - PubMed

Affiliation: DISPAA, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino, Italy ; HSO-USB, ESTEC, European Space Agency, Keplerlaan 1, 2200 AG Noordwijk, The Netherlands.

ABSTRACT
Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

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Related in: MedlinePlus

Timeline of burst appearance (T1) after the onset of microgravity (T0) during a parabola.
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fig5: Timeline of burst appearance (T1) after the onset of microgravity (T0) during a parabola.

Mentions: For each parabola, the average detection time (T1) of the first oxygen peaks from the start of the microgravity period (g < 1, T0; see Figure 5) has been calculated. The average time for all the parabolas is 2.4 secs. In order to evaluate a difference in the appearance of the first peak during the flight, the parabolas have been separated in three different groups (Table 1). Each group was composed of 10 parabolas. The objective was to investigate if a sort of “memory effect” of the stimulus during the repeated parabolas could cause a different response during the time of the flight (higher/lower, anticipated/retarded). The results showed a reduction, but not statistically significant, in the onset of the first peak during the last set of parabolas.


Oxidative stress and NO signalling in the root apex as an early response to changes in gravity conditions.

Mugnai S, Pandolfi C, Masi E, Azzarello E, Monetti E, Comparini D, Voigt B, Volkmann D, Mancuso S - Biomed Res Int (2014)

Timeline of burst appearance (T1) after the onset of microgravity (T0) during a parabola.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150467&req=5

fig5: Timeline of burst appearance (T1) after the onset of microgravity (T0) during a parabola.
Mentions: For each parabola, the average detection time (T1) of the first oxygen peaks from the start of the microgravity period (g < 1, T0; see Figure 5) has been calculated. The average time for all the parabolas is 2.4 secs. In order to evaluate a difference in the appearance of the first peak during the flight, the parabolas have been separated in three different groups (Table 1). Each group was composed of 10 parabolas. The objective was to investigate if a sort of “memory effect” of the stimulus during the repeated parabolas could cause a different response during the time of the flight (higher/lower, anticipated/retarded). The results showed a reduction, but not statistically significant, in the onset of the first peak during the last set of parabolas.

Bottom Line: The same results were obtained by ROS measurement.The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated.Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

View Article: PubMed Central - PubMed

Affiliation: DISPAA, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino, Italy ; HSO-USB, ESTEC, European Space Agency, Keplerlaan 1, 2200 AG Noordwijk, The Netherlands.

ABSTRACT
Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

Show MeSH
Related in: MedlinePlus