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Oxidative stress and NO signalling in the root apex as an early response to changes in gravity conditions.

Mugnai S, Pandolfi C, Masi E, Azzarello E, Monetti E, Comparini D, Voigt B, Volkmann D, Mancuso S - Biomed Res Int (2014)

Bottom Line: The same results were obtained by ROS measurement.The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated.Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

View Article: PubMed Central - PubMed

Affiliation: DISPAA, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino, Italy ; HSO-USB, ESTEC, European Space Agency, Keplerlaan 1, 2200 AG Noordwijk, The Netherlands.

ABSTRACT
Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

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Related in: MedlinePlus

Timeline of the NO production in a maize root apex in the three different constituent zones after gravistimulation (time = 0).
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fig3: Timeline of the NO production in a maize root apex in the three different constituent zones after gravistimulation (time = 0).

Mentions: Gravistimulation also promoted a very fast NO production from the root apex (Figure 3). A burst of NO was suddenly produced after only 2-3 seconds from gravistimulation, reaching a peak of 10 nM and lasting approximately 8 s before returning to the steady-state values in the TZ. Only a small and negligible efflux of NO was detectable in the DZ, and, importantly, NO bursts were not detected in the EZ region.


Oxidative stress and NO signalling in the root apex as an early response to changes in gravity conditions.

Mugnai S, Pandolfi C, Masi E, Azzarello E, Monetti E, Comparini D, Voigt B, Volkmann D, Mancuso S - Biomed Res Int (2014)

Timeline of the NO production in a maize root apex in the three different constituent zones after gravistimulation (time = 0).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150467&req=5

fig3: Timeline of the NO production in a maize root apex in the three different constituent zones after gravistimulation (time = 0).
Mentions: Gravistimulation also promoted a very fast NO production from the root apex (Figure 3). A burst of NO was suddenly produced after only 2-3 seconds from gravistimulation, reaching a peak of 10 nM and lasting approximately 8 s before returning to the steady-state values in the TZ. Only a small and negligible efflux of NO was detectable in the DZ, and, importantly, NO bursts were not detected in the EZ region.

Bottom Line: The same results were obtained by ROS measurement.The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated.Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

View Article: PubMed Central - PubMed

Affiliation: DISPAA, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino, Italy ; HSO-USB, ESTEC, European Space Agency, Keplerlaan 1, 2200 AG Noordwijk, The Netherlands.

ABSTRACT
Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution.

Show MeSH
Related in: MedlinePlus