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Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

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Related in: MedlinePlus

Effect of the combined treatment with all the seven supplement's components on IκBα phosphorylation and p65 expression in HaCaT keratinocytes. Cells were exposed to the combined treatment (CT) in the absence or in the presence of TNF-α (20 ng/mL) for 5 h. (a) A representative Western Blot of three similar experiments with the corresponding p-IκBα/IκBα ratio is shown. (b) A representative Western Blot of three similar experiments with the corresponding p65/α-actinin ratio is shown.
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fig9: Effect of the combined treatment with all the seven supplement's components on IκBα phosphorylation and p65 expression in HaCaT keratinocytes. Cells were exposed to the combined treatment (CT) in the absence or in the presence of TNF-α (20 ng/mL) for 5 h. (a) A representative Western Blot of three similar experiments with the corresponding p-IκBα/IκBα ratio is shown. (b) A representative Western Blot of three similar experiments with the corresponding p65/α-actinin ratio is shown.

Mentions: Afterwards, it was investigated if the combination could regulate the Nuclear Factor-κB (NF-κB) pathway, known to be involved in the synthesis of proinflammatory cytokines (Figure 9). In resting conditions, NF-κB is bound to its inhibitor IκBα in a nonphosphorylated form in the cytosol. When, following an inflammatory stimulus such as TNF-α, IκBα is phosphorylated and then degraded, NF-κB can translocate into the nucleus and activate the transcription of genes encoding proinflammatory cytokines. In agreement, the phosphorylation of IκBα (p-IκBα) increased in HaCaT cells treated with TNF-α. In line with the ability of the combined treatment to inhibit the production of IL-6 and MCP-1, it reduced the phosphorylation of IκBα (Figure 9(a)) in HaCaT cells, both in the presence and in the absence of TNF-α. Moreover, in the same conditions, it inhibited also the expression of the NF-κB subunit p65, decreasing the amount of NF-κB available for cytokine transcription.


Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Effect of the combined treatment with all the seven supplement's components on IκBα phosphorylation and p65 expression in HaCaT keratinocytes. Cells were exposed to the combined treatment (CT) in the absence or in the presence of TNF-α (20 ng/mL) for 5 h. (a) A representative Western Blot of three similar experiments with the corresponding p-IκBα/IκBα ratio is shown. (b) A representative Western Blot of three similar experiments with the corresponding p65/α-actinin ratio is shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150458&req=5

fig9: Effect of the combined treatment with all the seven supplement's components on IκBα phosphorylation and p65 expression in HaCaT keratinocytes. Cells were exposed to the combined treatment (CT) in the absence or in the presence of TNF-α (20 ng/mL) for 5 h. (a) A representative Western Blot of three similar experiments with the corresponding p-IκBα/IκBα ratio is shown. (b) A representative Western Blot of three similar experiments with the corresponding p65/α-actinin ratio is shown.
Mentions: Afterwards, it was investigated if the combination could regulate the Nuclear Factor-κB (NF-κB) pathway, known to be involved in the synthesis of proinflammatory cytokines (Figure 9). In resting conditions, NF-κB is bound to its inhibitor IκBα in a nonphosphorylated form in the cytosol. When, following an inflammatory stimulus such as TNF-α, IκBα is phosphorylated and then degraded, NF-κB can translocate into the nucleus and activate the transcription of genes encoding proinflammatory cytokines. In agreement, the phosphorylation of IκBα (p-IκBα) increased in HaCaT cells treated with TNF-α. In line with the ability of the combined treatment to inhibit the production of IL-6 and MCP-1, it reduced the phosphorylation of IκBα (Figure 9(a)) in HaCaT cells, both in the presence and in the absence of TNF-α. Moreover, in the same conditions, it inhibited also the expression of the NF-κB subunit p65, decreasing the amount of NF-κB available for cytokine transcription.

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

Show MeSH
Related in: MedlinePlus