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Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

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Related in: MedlinePlus

Effect of the seven supplement's components on HaCaT keratinocyte MCP-1 production. Cells were exposed to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of a number of determinations ranging from 4 to 6. a: significantly different from control (P < 0.05); b: significantly different from control (P < 0.01), one-way ANOVA followed by Dunnett's test. (c) Cells were exposed simultaneously to all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 6. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test). (d) The cells were exposed simultaneously to all the components excluding Se, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiments. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 6 to 8. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test).
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fig8: Effect of the seven supplement's components on HaCaT keratinocyte MCP-1 production. Cells were exposed to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of a number of determinations ranging from 4 to 6. a: significantly different from control (P < 0.05); b: significantly different from control (P < 0.01), one-way ANOVA followed by Dunnett's test. (c) Cells were exposed simultaneously to all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 6. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test). (d) The cells were exposed simultaneously to all the components excluding Se, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiments. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 6 to 8. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test).

Mentions: Data (expressed as the means ± SE) were analyzed by either two-tailed unpaired t-test (Figures 1, 4, 5, 7(c), 8(c), and 8(d)) when two groups were compared or by one-way analysis of variance (ANOVA) followed by Dunnett's test when three or more groups were compared (Figures 2, 3, 6, 7(a), 7(b), 8(a), and 8(b)) (InStat GraphPad software).


Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Effect of the seven supplement's components on HaCaT keratinocyte MCP-1 production. Cells were exposed to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of a number of determinations ranging from 4 to 6. a: significantly different from control (P < 0.05); b: significantly different from control (P < 0.01), one-way ANOVA followed by Dunnett's test. (c) Cells were exposed simultaneously to all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 6. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test). (d) The cells were exposed simultaneously to all the components excluding Se, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiments. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 6 to 8. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4150458&req=5

fig8: Effect of the seven supplement's components on HaCaT keratinocyte MCP-1 production. Cells were exposed to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of a number of determinations ranging from 4 to 6. a: significantly different from control (P < 0.05); b: significantly different from control (P < 0.01), one-way ANOVA followed by Dunnett's test. (c) Cells were exposed simultaneously to all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 6. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test). (d) The cells were exposed simultaneously to all the components excluding Se, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiments. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 6 to 8. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test).
Mentions: Data (expressed as the means ± SE) were analyzed by either two-tailed unpaired t-test (Figures 1, 4, 5, 7(c), 8(c), and 8(d)) when two groups were compared or by one-way analysis of variance (ANOVA) followed by Dunnett's test when three or more groups were compared (Figures 2, 3, 6, 7(a), 7(b), 8(a), and 8(b)) (InStat GraphPad software).

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

Show MeSH
Related in: MedlinePlus