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Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

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Related in: MedlinePlus

Effect of the combined treatment with all the seven supplement's components on ROS production in keratinocytes exposed or not to H2O2. Cells were treated simultaneously for 24 h with all the components and then exposed to H2O2 (100 μM, 30 min) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 8. Significance was evaluated by the two-tailed unpaired t-test.
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fig4: Effect of the combined treatment with all the seven supplement's components on ROS production in keratinocytes exposed or not to H2O2. Cells were treated simultaneously for 24 h with all the components and then exposed to H2O2 (100 μM, 30 min) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 8. Significance was evaluated by the two-tailed unpaired t-test.

Mentions: Data (expressed as the means ± SE) were analyzed by either two-tailed unpaired t-test (Figures 1, 4, 5, 7(c), 8(c), and 8(d)) when two groups were compared or by one-way analysis of variance (ANOVA) followed by Dunnett's test when three or more groups were compared (Figures 2, 3, 6, 7(a), 7(b), 8(a), and 8(b)) (InStat GraphPad software).


Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.

Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, Cittadini A, Calviello G - Biomed Res Int (2014)

Effect of the combined treatment with all the seven supplement's components on ROS production in keratinocytes exposed or not to H2O2. Cells were treated simultaneously for 24 h with all the components and then exposed to H2O2 (100 μM, 30 min) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 8. Significance was evaluated by the two-tailed unpaired t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150458&req=5

fig4: Effect of the combined treatment with all the seven supplement's components on ROS production in keratinocytes exposed or not to H2O2. Cells were treated simultaneously for 24 h with all the components and then exposed to H2O2 (100 μM, 30 min) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 8. Significance was evaluated by the two-tailed unpaired t-test.
Mentions: Data (expressed as the means ± SE) were analyzed by either two-tailed unpaired t-test (Figures 1, 4, 5, 7(c), 8(c), and 8(d)) when two groups were compared or by one-way analysis of variance (ANOVA) followed by Dunnett's test when three or more groups were compared (Figures 2, 3, 6, 7(a), 7(b), 8(a), and 8(b)) (InStat GraphPad software).

Bottom Line: Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated.Moreover, the combination showed remarkable anti-inflammatory properties.It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.

ABSTRACT
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.

Show MeSH
Related in: MedlinePlus