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Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.

Hu YY, Dong WD, Xu YF, Yao XD, Peng B, Liu M, Zheng JH - Biomed Res Int (2014)

Bottom Line: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers.Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.

ABSTRACT

Background: Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.

Methods: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.

Results: The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).

Conclusion: Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.

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Relation between urinary levels of miR-155 and (a) estimated glomerular filtration (eGFR) and (b) CRP. Data are compared by Spearman's rank correlation coefficient.
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fig2: Relation between urinary levels of miR-155 and (a) estimated glomerular filtration (eGFR) and (b) CRP. Data are compared by Spearman's rank correlation coefficient.

Mentions: Since only 30 healthy controls provided us with the serum samples for detection of miR-155, however, all patients and healthy controls donated urine samples for study; it seems that the detection of urine samples is noninvasive and more popular and acceptable by subjects. We analyzed correlations between urinary miR-155 levels and clinical parameters including C-reactive protein, estimated glomerular filtration (eGFR). Urinary levels of miR-155 significantly and inversely correlate with the eGFR (r = −0.432, P < 0.01), and urinary levels of miR-155 positively correlate with serum CRP (r = 0.266, P < 0.01); the details were shown in Figure 2.


Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.

Hu YY, Dong WD, Xu YF, Yao XD, Peng B, Liu M, Zheng JH - Biomed Res Int (2014)

Relation between urinary levels of miR-155 and (a) estimated glomerular filtration (eGFR) and (b) CRP. Data are compared by Spearman's rank correlation coefficient.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150454&req=5

fig2: Relation between urinary levels of miR-155 and (a) estimated glomerular filtration (eGFR) and (b) CRP. Data are compared by Spearman's rank correlation coefficient.
Mentions: Since only 30 healthy controls provided us with the serum samples for detection of miR-155, however, all patients and healthy controls donated urine samples for study; it seems that the detection of urine samples is noninvasive and more popular and acceptable by subjects. We analyzed correlations between urinary miR-155 levels and clinical parameters including C-reactive protein, estimated glomerular filtration (eGFR). Urinary levels of miR-155 significantly and inversely correlate with the eGFR (r = −0.432, P < 0.01), and urinary levels of miR-155 positively correlate with serum CRP (r = 0.266, P < 0.01); the details were shown in Figure 2.

Bottom Line: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers.Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.

ABSTRACT

Background: Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.

Methods: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.

Results: The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).

Conclusion: Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.

Show MeSH
Related in: MedlinePlus