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Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.

Hu YY, Dong WD, Xu YF, Yao XD, Peng B, Liu M, Zheng JH - Biomed Res Int (2014)

Bottom Line: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers.Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.

ABSTRACT

Background: Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.

Methods: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.

Results: The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).

Conclusion: Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.

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Comparison of serum (a) and urinary (b) levels of miR-155 between patients with nephrolithiasis and normal controls. Data are compared by Mann-Whitney U test. Levels are represented as ratio to the average of controls.
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fig1: Comparison of serum (a) and urinary (b) levels of miR-155 between patients with nephrolithiasis and normal controls. Data are compared by Mann-Whitney U test. Levels are represented as ratio to the average of controls.

Mentions: As compared to healthy control, the serum miR-155 levels were significantly higher in nephrolithiasis patients (3.83, IQR from 2.55 to 8.29) versus 1.67, IQR from 0.43 to 3.24, P < 0.01 (Figure 1(a)). Similarly, nephrolithiasis patients group had a significantly higher urinary levels of miR-155 (3.36, IQR from 1.69 to 6.02 versus 0.99, IQR from 0.76 to 1.44, P < 0.01) than healthy cohorts (Figure 1(b)).


Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.

Hu YY, Dong WD, Xu YF, Yao XD, Peng B, Liu M, Zheng JH - Biomed Res Int (2014)

Comparison of serum (a) and urinary (b) levels of miR-155 between patients with nephrolithiasis and normal controls. Data are compared by Mann-Whitney U test. Levels are represented as ratio to the average of controls.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150454&req=5

fig1: Comparison of serum (a) and urinary (b) levels of miR-155 between patients with nephrolithiasis and normal controls. Data are compared by Mann-Whitney U test. Levels are represented as ratio to the average of controls.
Mentions: As compared to healthy control, the serum miR-155 levels were significantly higher in nephrolithiasis patients (3.83, IQR from 2.55 to 8.29) versus 1.67, IQR from 0.43 to 3.24, P < 0.01 (Figure 1(a)). Similarly, nephrolithiasis patients group had a significantly higher urinary levels of miR-155 (3.36, IQR from 1.69 to 6.02 versus 0.99, IQR from 0.76 to 1.44, P < 0.01) than healthy cohorts (Figure 1(b)).

Bottom Line: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers.Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.

ABSTRACT

Background: Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.

Methods: Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.

Results: The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).

Conclusion: Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.

Show MeSH
Related in: MedlinePlus