Limits...
Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats.

Yuan Z, Zhang J, Yang C - Evid Based Complement Alternat Med (2014)

Bottom Line: This study investigated the effects of Ligusticum wallichii on IL-1β expression in myocardium and central nervous system after AMI.AMI rat was administrated with Ligusticum wallichii extract.Ligusticum wallichii extract reduced the expression of IL-1β in myocardium by regulating TLR4-NF-κB signaling pathway and inhibited IL-1β in hypothalamus by regulating NPY mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 314 An Shan Xi Road, Nan Kai District, Tianjin 300193, China.

ABSTRACT
This study investigated the effects of Ligusticum wallichii on IL-1β expression in myocardium and central nervous system after AMI. AMI rat was administrated with Ligusticum wallichii extract. A series of assays were used to detect the effects of Ligusticum wallichii extract on infarct size, left ventricular ejection fraction, expression of TLR-4, NF-κB, and IL-1β in myocardium, IL-1β expression in serum and hypothalamus, and NPY expression in hypothalamus. We observed that Ligusticum wallichii extract improved the left ventricular ejection fraction and reduced infarct area enlargement after AMI, by inhibiting the expression of IL-1β in myocardium, serum, and hypothalamus. Ligusticum wallichii extract reduced the expression of IL-1β in myocardium by regulating TLR4-NF-κB signaling pathway and inhibited IL-1β in hypothalamus by regulating NPY mRNA expression.

No MeSH data available.


Related in: MedlinePlus

Ligusticum wallichii extract inhibited the expression of TLR4, TRAF-6, NF-κB in myocardium. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were TLR4 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TLR4-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited TLR4 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (c) The brown areas were TRAF-6 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (d) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TRAF-6-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii and aspirin both inhibited TRAF-6 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (e) The brown areas were NF-κB positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (f) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of NF-κB-like immunoreactive cells increased significantly in the cytoplasm and nucleus after AMI. Ligusticum wallichii and aspirin both inhibited NF-κB protein expression versus AMI group (*P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4150451&req=5

fig4: Ligusticum wallichii extract inhibited the expression of TLR4, TRAF-6, NF-κB in myocardium. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were TLR4 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TLR4-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited TLR4 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (c) The brown areas were TRAF-6 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (d) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TRAF-6-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii and aspirin both inhibited TRAF-6 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (e) The brown areas were NF-κB positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (f) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of NF-κB-like immunoreactive cells increased significantly in the cytoplasm and nucleus after AMI. Ligusticum wallichii and aspirin both inhibited NF-κB protein expression versus AMI group (*P < 0.05).

Mentions: TLR4 (Figures 4(a) and 4(b)) and TRAF-6 (Figures 4(c), 4(d)) immunohistochemical staining could be detected to the cells of rat myocardium after AMI. The TLR4 and TRAF-6 protein was predominantly expressed in the cytoplasm. Few cells positive for TLR4-like and immunoreaction were observed in the sham group. Intensive TLR4-like and TRAF-6-like immunostaining was present in myocardium after AMI. Significant changes of TLR4 and TRAF-6 protein expression were observed in the Ligusticum wallichii group and aspirin group.


Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats.

Yuan Z, Zhang J, Yang C - Evid Based Complement Alternat Med (2014)

Ligusticum wallichii extract inhibited the expression of TLR4, TRAF-6, NF-κB in myocardium. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were TLR4 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TLR4-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited TLR4 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (c) The brown areas were TRAF-6 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (d) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TRAF-6-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii and aspirin both inhibited TRAF-6 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (e) The brown areas were NF-κB positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (f) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of NF-κB-like immunoreactive cells increased significantly in the cytoplasm and nucleus after AMI. Ligusticum wallichii and aspirin both inhibited NF-κB protein expression versus AMI group (*P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150451&req=5

fig4: Ligusticum wallichii extract inhibited the expression of TLR4, TRAF-6, NF-κB in myocardium. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were TLR4 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TLR4-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited TLR4 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (c) The brown areas were TRAF-6 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (d) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TRAF-6-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group (**P < 0.01). Ligusticum wallichii and aspirin both inhibited TRAF-6 protein expression versus AMI group (*P < 0.05), but there were no remarkable differences between LW group and aspirin group. (e) The brown areas were NF-κB positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (f) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of NF-κB-like immunoreactive cells increased significantly in the cytoplasm and nucleus after AMI. Ligusticum wallichii and aspirin both inhibited NF-κB protein expression versus AMI group (*P < 0.05).
Mentions: TLR4 (Figures 4(a) and 4(b)) and TRAF-6 (Figures 4(c), 4(d)) immunohistochemical staining could be detected to the cells of rat myocardium after AMI. The TLR4 and TRAF-6 protein was predominantly expressed in the cytoplasm. Few cells positive for TLR4-like and immunoreaction were observed in the sham group. Intensive TLR4-like and TRAF-6-like immunostaining was present in myocardium after AMI. Significant changes of TLR4 and TRAF-6 protein expression were observed in the Ligusticum wallichii group and aspirin group.

Bottom Line: This study investigated the effects of Ligusticum wallichii on IL-1β expression in myocardium and central nervous system after AMI.AMI rat was administrated with Ligusticum wallichii extract.Ligusticum wallichii extract reduced the expression of IL-1β in myocardium by regulating TLR4-NF-κB signaling pathway and inhibited IL-1β in hypothalamus by regulating NPY mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 314 An Shan Xi Road, Nan Kai District, Tianjin 300193, China.

ABSTRACT
This study investigated the effects of Ligusticum wallichii on IL-1β expression in myocardium and central nervous system after AMI. AMI rat was administrated with Ligusticum wallichii extract. A series of assays were used to detect the effects of Ligusticum wallichii extract on infarct size, left ventricular ejection fraction, expression of TLR-4, NF-κB, and IL-1β in myocardium, IL-1β expression in serum and hypothalamus, and NPY expression in hypothalamus. We observed that Ligusticum wallichii extract improved the left ventricular ejection fraction and reduced infarct area enlargement after AMI, by inhibiting the expression of IL-1β in myocardium, serum, and hypothalamus. Ligusticum wallichii extract reduced the expression of IL-1β in myocardium by regulating TLR4-NF-κB signaling pathway and inhibited IL-1β in hypothalamus by regulating NPY mRNA expression.

No MeSH data available.


Related in: MedlinePlus