Limits...
Antibacterial activity of Pseudonocardia sp. JB05, a rare salty soil actinomycete against Staphylococcus aureus.

Jafari N, Behroozi R, Farajzadeh D, Farsi M, Akbari-Noghabi K - Biomed Res Int (2014)

Bottom Line: Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy.JB05, based on the results of biochemical and 16S rDNA gene sequence analysis.The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward the Pseudonocardia scale-up for the production and identification of the antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran ; Department of Plant Biotechnology, Buali-Sina University of Hamedan, Iran.

ABSTRACT
Staphylococcus aureus is a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds against S. aureus and characterize the structure of the resultant antibacterial compounds. Therefore, a slightly modified agar well diffusion method was used to determine the antibacterial activity of actinomycete isolates against the test microorganisms. The bacterial extracts with antibacterial activity were fractionated by silica gel and G-25 sephadex column chromatography. Also, the active fractions were analyzed by thin layer chromatography. Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy. One of the isolates, which had a broad spectrum and high antibacterial activity, was designated as Pseudonocardia sp. JB05, based on the results of biochemical and 16S rDNA gene sequence analysis. Minimum inhibitory concentration for this bacterium was 40 AU mL(-1) against S. aureus. The antibacterial activity of this bacterium was stable after autoclaving, 10% SDS, boiling, and proteinase K. Thin layer chromatography, using anthrone reagent, showed the presence of carbohydrates in the purified antibacterial compound. Finally, FT-IR spectrum of the active compound illustrated hydroxyl groups, hydrocarbon skeleton, and double bond of polygenic compounds in its structure. To the best of our knowledge, this is the first report describing the efficient antibacterial activity by a local strain of Pseudonocardia. The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward the Pseudonocardia scale-up for the production and identification of the antibacterial compounds.

Show MeSH

Related in: MedlinePlus

Screening of actinomycete isolates based on (a) their antibacterial activity against S. aureus and (b) their 16S rDNA gene fragments. From right to left: gel electrophoresis of 1 kb DNA ladder and the PCR products of 16S rDNA gene fragments. Numbers shown above each lane or on each plate are the numbers of the actinomycete isolates.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150441&req=5

fig1: Screening of actinomycete isolates based on (a) their antibacterial activity against S. aureus and (b) their 16S rDNA gene fragments. From right to left: gel electrophoresis of 1 kb DNA ladder and the PCR products of 16S rDNA gene fragments. Numbers shown above each lane or on each plate are the numbers of the actinomycete isolates.

Mentions: Antibacterial activity assay was performed using well diffusion method. Among the sixty isolated actinomycetes, only four strains (number 012-1, number 012-2, number 010-31, and number 025-26) showed antibacterial activity against at least one test microorganisms (Figure 1(a)). Strain number 010-31 had the highest inhibitory effect on the growth of all tested pathogenic bacteria, especially on S. aureus (Table 1). pH and temperature ranges for the growth of isolate number 010-31 were 6–10 and 27–32°C, respectively, with an optimal growth on pH 8 and 30°C, respectively. It was also capable to grow in WYE medium containing 5% w/v NaCl.


Antibacterial activity of Pseudonocardia sp. JB05, a rare salty soil actinomycete against Staphylococcus aureus.

Jafari N, Behroozi R, Farajzadeh D, Farsi M, Akbari-Noghabi K - Biomed Res Int (2014)

Screening of actinomycete isolates based on (a) their antibacterial activity against S. aureus and (b) their 16S rDNA gene fragments. From right to left: gel electrophoresis of 1 kb DNA ladder and the PCR products of 16S rDNA gene fragments. Numbers shown above each lane or on each plate are the numbers of the actinomycete isolates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150441&req=5

fig1: Screening of actinomycete isolates based on (a) their antibacterial activity against S. aureus and (b) their 16S rDNA gene fragments. From right to left: gel electrophoresis of 1 kb DNA ladder and the PCR products of 16S rDNA gene fragments. Numbers shown above each lane or on each plate are the numbers of the actinomycete isolates.
Mentions: Antibacterial activity assay was performed using well diffusion method. Among the sixty isolated actinomycetes, only four strains (number 012-1, number 012-2, number 010-31, and number 025-26) showed antibacterial activity against at least one test microorganisms (Figure 1(a)). Strain number 010-31 had the highest inhibitory effect on the growth of all tested pathogenic bacteria, especially on S. aureus (Table 1). pH and temperature ranges for the growth of isolate number 010-31 were 6–10 and 27–32°C, respectively, with an optimal growth on pH 8 and 30°C, respectively. It was also capable to grow in WYE medium containing 5% w/v NaCl.

Bottom Line: Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy.JB05, based on the results of biochemical and 16S rDNA gene sequence analysis.The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward the Pseudonocardia scale-up for the production and identification of the antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran ; Department of Plant Biotechnology, Buali-Sina University of Hamedan, Iran.

ABSTRACT
Staphylococcus aureus is a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds against S. aureus and characterize the structure of the resultant antibacterial compounds. Therefore, a slightly modified agar well diffusion method was used to determine the antibacterial activity of actinomycete isolates against the test microorganisms. The bacterial extracts with antibacterial activity were fractionated by silica gel and G-25 sephadex column chromatography. Also, the active fractions were analyzed by thin layer chromatography. Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy. One of the isolates, which had a broad spectrum and high antibacterial activity, was designated as Pseudonocardia sp. JB05, based on the results of biochemical and 16S rDNA gene sequence analysis. Minimum inhibitory concentration for this bacterium was 40 AU mL(-1) against S. aureus. The antibacterial activity of this bacterium was stable after autoclaving, 10% SDS, boiling, and proteinase K. Thin layer chromatography, using anthrone reagent, showed the presence of carbohydrates in the purified antibacterial compound. Finally, FT-IR spectrum of the active compound illustrated hydroxyl groups, hydrocarbon skeleton, and double bond of polygenic compounds in its structure. To the best of our knowledge, this is the first report describing the efficient antibacterial activity by a local strain of Pseudonocardia. The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward the Pseudonocardia scale-up for the production and identification of the antibacterial compounds.

Show MeSH
Related in: MedlinePlus