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Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus

Effects of crocin or E2 on oxidative stress status including (a) MDA and (b) SOD in serum and (c) NOX4 and (d) MnSOD expression in bone tissue of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
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fig7: Effects of crocin or E2 on oxidative stress status including (a) MDA and (b) SOD in serum and (c) NOX4 and (d) MnSOD expression in bone tissue of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.

Mentions: The serum ROS level and antioxidative statuses of the different experimental groups are presented in Figures 7(a)-7(b). The MDA level demonstrated a significant 116.1% increase compared to the sham group (P < 0.05). In contrast, the total SOD activity significantly decreased by 47.3% in response to OVX for 16 weeks compared to the sham group (P < 0.05). In order to further evaluate the potential sources of the radicals and the mitochondrial status, the protein expressions of NOX4 and MnSOD in bone tissue were detected using western blot. The data (Figures 7(c)–7(d)) showed that OVX caused significant increase in the expression of NOX4 and decrease in MnSOD compared with that in sham group (both P < 0.05). All crocin doses significantly decreased serum MDA content and bone NOX4 expression and stimulated SOD activity in both serum and bone tissue of OVX rats (all P < 0.05) in a dose-dependent manner. E2 also significantly exerted similar antioxidative effect to that of H-C crocin (all P > 0.05).


Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Effects of crocin or E2 on oxidative stress status including (a) MDA and (b) SOD in serum and (c) NOX4 and (d) MnSOD expression in bone tissue of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150429&req=5

fig7: Effects of crocin or E2 on oxidative stress status including (a) MDA and (b) SOD in serum and (c) NOX4 and (d) MnSOD expression in bone tissue of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
Mentions: The serum ROS level and antioxidative statuses of the different experimental groups are presented in Figures 7(a)-7(b). The MDA level demonstrated a significant 116.1% increase compared to the sham group (P < 0.05). In contrast, the total SOD activity significantly decreased by 47.3% in response to OVX for 16 weeks compared to the sham group (P < 0.05). In order to further evaluate the potential sources of the radicals and the mitochondrial status, the protein expressions of NOX4 and MnSOD in bone tissue were detected using western blot. The data (Figures 7(c)–7(d)) showed that OVX caused significant increase in the expression of NOX4 and decrease in MnSOD compared with that in sham group (both P < 0.05). All crocin doses significantly decreased serum MDA content and bone NOX4 expression and stimulated SOD activity in both serum and bone tissue of OVX rats (all P < 0.05) in a dose-dependent manner. E2 also significantly exerted similar antioxidative effect to that of H-C crocin (all P > 0.05).

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus