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Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus

Effects of crocin or E2 on biochemical parameters including (a) S-Ca, (b) S-P, (c) U-Ca/Cr, (d) U-P/Cr, (e) ALP, and (f) OC in serum and urine of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
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fig6: Effects of crocin or E2 on biochemical parameters including (a) S-Ca, (b) S-P, (c) U-Ca/Cr, (d) U-P/Cr, (e) ALP, and (f) OC in serum and urine of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.

Mentions: Date presented in Figure 6 show the effects of crocin or E2 on biochemical parameters in the serum and urine of OVX rats. There were no significant differences in the values for S-Ca or S-P among all of the study groups (Figures 6(a)-6(b); both P > 0.05). However, the levels of U-Ca/Cr, U-P/Cr, ALP, and OC (Figures 6(c)–6(f)) were significantly increased in the OVX group by 104.5%, 36.9%, 105.9%, and 48.5% compared to the sham group (all P < 0.05), respectively. All three crocin doses significantly prevented the increases in U-Ca/Cr and ALP levels (all P < 0.05) in a dose-dependent manner. Higher dosage crocin treatment (10 or 20 mg/kg/day) significantly prevented the increases in U-P/Cr and OC levels (both P < 0.05). Again, E2 administration also reversed the above-mentioned increases, which were statistically significant. It is worth noting that the reductions in U-P/Cr, ALP, and OC in the H-C crocin group were similar to those observed for E2 (all P > 0.05).


Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Effects of crocin or E2 on biochemical parameters including (a) S-Ca, (b) S-P, (c) U-Ca/Cr, (d) U-P/Cr, (e) ALP, and (f) OC in serum and urine of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150429&req=5

fig6: Effects of crocin or E2 on biochemical parameters including (a) S-Ca, (b) S-P, (c) U-Ca/Cr, (d) U-P/Cr, (e) ALP, and (f) OC in serum and urine of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
Mentions: Date presented in Figure 6 show the effects of crocin or E2 on biochemical parameters in the serum and urine of OVX rats. There were no significant differences in the values for S-Ca or S-P among all of the study groups (Figures 6(a)-6(b); both P > 0.05). However, the levels of U-Ca/Cr, U-P/Cr, ALP, and OC (Figures 6(c)–6(f)) were significantly increased in the OVX group by 104.5%, 36.9%, 105.9%, and 48.5% compared to the sham group (all P < 0.05), respectively. All three crocin doses significantly prevented the increases in U-Ca/Cr and ALP levels (all P < 0.05) in a dose-dependent manner. Higher dosage crocin treatment (10 or 20 mg/kg/day) significantly prevented the increases in U-P/Cr and OC levels (both P < 0.05). Again, E2 administration also reversed the above-mentioned increases, which were statistically significant. It is worth noting that the reductions in U-P/Cr, ALP, and OC in the H-C crocin group were similar to those observed for E2 (all P > 0.05).

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus