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Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus

3D reconstruction images of VOI in L4 vertebrae showed the effects of crocin or E2 treatments on cancellous bone microarchitecture in OVX rats.
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Related In: Results  -  Collection


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fig4: 3D reconstruction images of VOI in L4 vertebrae showed the effects of crocin or E2 treatments on cancellous bone microarchitecture in OVX rats.

Mentions: Three-dimensional reconstruction images of the L4 vertebrae showed differences in trabecular microarchitecture among the various treatment groups as represented in Figure 4. Analysis of the representative samples (Figure 5) indicated that OVX resulted in the deterioration of the trabecular bone microarchitecture, as demonstrated by the reduced BV/TV, Tb.N, Tb.Th, and Conn.D, compared with the sham group (all P < 0.05). In contrast, Tb.Sp and SMI were significantly increased in response to OVX compared to the sham group (both P < 0.05). Higher dosage crocin treatment (10 or 20 mg/kg/day) significantly improved the microarchitecture deterioration mentioned above (all P < 0.05), while E2 also reversed these parameters to the similar degree as that in the H-C group (all P > 0.05).


Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

3D reconstruction images of VOI in L4 vertebrae showed the effects of crocin or E2 treatments on cancellous bone microarchitecture in OVX rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150429&req=5

fig4: 3D reconstruction images of VOI in L4 vertebrae showed the effects of crocin or E2 treatments on cancellous bone microarchitecture in OVX rats.
Mentions: Three-dimensional reconstruction images of the L4 vertebrae showed differences in trabecular microarchitecture among the various treatment groups as represented in Figure 4. Analysis of the representative samples (Figure 5) indicated that OVX resulted in the deterioration of the trabecular bone microarchitecture, as demonstrated by the reduced BV/TV, Tb.N, Tb.Th, and Conn.D, compared with the sham group (all P < 0.05). In contrast, Tb.Sp and SMI were significantly increased in response to OVX compared to the sham group (both P < 0.05). Higher dosage crocin treatment (10 or 20 mg/kg/day) significantly improved the microarchitecture deterioration mentioned above (all P < 0.05), while E2 also reversed these parameters to the similar degree as that in the H-C group (all P > 0.05).

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus