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Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus

Effects of crocin or E2 on (a) body weight and (b) uterus index of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
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fig1: Effects of crocin or E2 on (a) body weight and (b) uterus index of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.

Mentions: As shown in Figure 1(a), there were no significant differences in the initial mean body weight between the six study groups (all P > 0.05). At week 4, the OVX group was 23.2% heavier than the sham group (P < 0.05), while E2 significantly decreased the body weight to a level similar to that of the sham group 8 weeks after the operation (P < 0.05). However, none of the three crocin doses had a significant effect in preventing the body weight increase at any time point. As shown in Figure 1(b), the uterus weight in the OVX group was significantly reduced to 21.1% of the uterus weight in the sham group (P < 0.05), indicating the success of the surgical procedure. E2 significantly prevented uterine weight loss compared to the OVX group (P < 0.05). However, none of the crocin dose levels elicited any uterotrophic effects.


Preventive effect of crocin on osteoporosis in an ovariectomized rat model.

Cao PC, Xiao WX, Yan YB, Zhao X, Liu S, Feng J, Zhang W, Wang J, Feng YF, Lei W - Evid Based Complement Alternat Med (2014)

Effects of crocin or E2 on (a) body weight and (b) uterus index of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4150429&req=5

fig1: Effects of crocin or E2 on (a) body weight and (b) uterus index of OVX rats. Values are mean ± SD, n = 10. *P < 0.05 versus sham; #P < 0.05 versus OVX.
Mentions: As shown in Figure 1(a), there were no significant differences in the initial mean body weight between the six study groups (all P > 0.05). At week 4, the OVX group was 23.2% heavier than the sham group (P < 0.05), while E2 significantly decreased the body weight to a level similar to that of the sham group 8 weeks after the operation (P < 0.05). However, none of the three crocin doses had a significant effect in preventing the body weight increase at any time point. As shown in Figure 1(b), the uterus weight in the OVX group was significantly reduced to 21.1% of the uterus weight in the sham group (P < 0.05), indicating the success of the surgical procedure. E2 significantly prevented uterine weight loss compared to the OVX group (P < 0.05). However, none of the crocin dose levels elicited any uterotrophic effects.

Bottom Line: Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers.Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue.In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 127, West Changle Road, Xi'an, Shaanxi 710032, China.

ABSTRACT
The purpose of this study was to investigate the therapeutic effects of crocin on ovariectomy-induced osteoporosis in rats. Female Sprague-Dawley rats were randomly assigned to a sham-operated group (sham) and five ovariectomy (OVX) subgroups, that is, OVX with vehicle (OVX), OVX with 17β-estradiol (E 2, 25 μg/kg/day), and OVX with graded crocin doses (5, 10, or 20 mg/kg/day). Daily oral administration of E 2 or crocin started 4 weeks after OVX and lasted for 16 weeks. Our results showed that crocin dose-dependently inhibited the BMD reduction of L4 vertebrae and femurs caused by OVX and prevented the deterioration of trabecular microarchitecture, which were accompanied by a significant decrease in skeletal remodeling as evidenced by the lower levels of bone turnover markers. Furthermore, crocin reversed the oxidative stress status in both serum and bone tissue. The present study indicates that the administration of crocin at higher doses over a 16-week period can prevent OVX-induced osteoporosis in rats without hyperplastic effects on the uterus, which may, at least partially, be attributed to crocin's antioxidative property. In brief, crocin is a natural alternative for postmenopausal osteoporosis treatment in elderly women.

No MeSH data available.


Related in: MedlinePlus