Limits...
Molecular cloning and characterization of a glycine-like receptor gene from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

Flores-Fernández JM, Gutiérrez-Ortega A, Padilla-Camberos E, Rosario-Cruz R, Hernández-Gutiérrez R, Martínez-Velázquez M - Parasite (2014)

Bottom Line: The polypeptide also contains the PAR motif, which is important for forming anion channels, and a conserved glycine residue at the third transmembrane domain, which is essential for high ivermectin sensitivity.PCR analyses showed that RmGlyR is expressed at egg, larval and adult developmental stages.Our findings suggest that the deduced receptor is an additional molecular target to ivermectin and it might be involved in ivermectin resistance in R. microplus.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, México.

Show MeSH
Expression of the RmGlyR gene in different stages of R. microplus. RT-PCR reactions were performed using total RNA from egg, larvae and adult tissues (ovary and midgut). Tick actin gene was used as a control. The data are expressed as a density ratio between RmGlyR and actin products.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150426&req=5

Figure 4: Expression of the RmGlyR gene in different stages of R. microplus. RT-PCR reactions were performed using total RNA from egg, larvae and adult tissues (ovary and midgut). Tick actin gene was used as a control. The data are expressed as a density ratio between RmGlyR and actin products.

Mentions: To determine the expression profiles of the RmGlyR gene, total RNA was extracted from tissues of different developmental stages, including eggs, larvae, and adult. RT-PCRs were performed using the primers F-RmGlyR and R-RmGlyR. The expression levels of RmGlyR were normalized by the actin levels, as mentioned before. The highest expression of RmGlyR was observed in adult ovary tissue, whereas at the egg stage the expression level was lower (Fig. 4).Figure 4.


Molecular cloning and characterization of a glycine-like receptor gene from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

Flores-Fernández JM, Gutiérrez-Ortega A, Padilla-Camberos E, Rosario-Cruz R, Hernández-Gutiérrez R, Martínez-Velázquez M - Parasite (2014)

Expression of the RmGlyR gene in different stages of R. microplus. RT-PCR reactions were performed using total RNA from egg, larvae and adult tissues (ovary and midgut). Tick actin gene was used as a control. The data are expressed as a density ratio between RmGlyR and actin products.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150426&req=5

Figure 4: Expression of the RmGlyR gene in different stages of R. microplus. RT-PCR reactions were performed using total RNA from egg, larvae and adult tissues (ovary and midgut). Tick actin gene was used as a control. The data are expressed as a density ratio between RmGlyR and actin products.
Mentions: To determine the expression profiles of the RmGlyR gene, total RNA was extracted from tissues of different developmental stages, including eggs, larvae, and adult. RT-PCRs were performed using the primers F-RmGlyR and R-RmGlyR. The expression levels of RmGlyR were normalized by the actin levels, as mentioned before. The highest expression of RmGlyR was observed in adult ovary tissue, whereas at the egg stage the expression level was lower (Fig. 4).Figure 4.

Bottom Line: The polypeptide also contains the PAR motif, which is important for forming anion channels, and a conserved glycine residue at the third transmembrane domain, which is essential for high ivermectin sensitivity.PCR analyses showed that RmGlyR is expressed at egg, larval and adult developmental stages.Our findings suggest that the deduced receptor is an additional molecular target to ivermectin and it might be involved in ivermectin resistance in R. microplus.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, México.

Show MeSH