Molecular cloning and characterization of a glycine-like receptor gene from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).
Bottom Line: The polypeptide also contains the PAR motif, which is important for forming anion channels, and a conserved glycine residue at the third transmembrane domain, which is essential for high ivermectin sensitivity.PCR analyses showed that RmGlyR is expressed at egg, larval and adult developmental stages.Our findings suggest that the deduced receptor is an additional molecular target to ivermectin and it might be involved in ivermectin resistance in R. microplus.
Affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, México.Show MeSH
Mentions: To determine the expression profiles of the RmGlyR gene, total RNA was extracted from tissues of different developmental stages, including eggs, larvae, and adult. RT-PCRs were performed using the primers F-RmGlyR and R-RmGlyR. The expression levels of RmGlyR were normalized by the actin levels, as mentioned before. The highest expression of RmGlyR was observed in adult ovary tissue, whereas at the egg stage the expression level was lower (Fig. 4).Figure 4.
Affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, México.