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Ultrastructure and development of Nosema podocotyloidis n. sp. (Microsporidia), a hyperparasite of Podocotyloides magnatestis (Trematoda), a parasite of Parapristipoma octolineatum (Teleostei).

Toguebaye BS, Quilichini Y, Diagne PM, Marchand B - Parasite (2014)

Bottom Line: The polaroplast is divided into two regions: an outer electron-dense cup with granular content and lacking lamellae and an internal region, less electron-dense, composed of irregularly arranged sacs.The posterior vacuole, with an amorphous electron-dense content, is present.The new species is compared with other species of Nosema from trematodes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Parasitologie, Faculté des Sciences et Techniques, BP 5055, Dakar, République du Sénégal.

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Nosema podocotyloidis n. sp. A. Young sporoblast showing numerous electron-lucent vesicles (arrows and insert) and the endoplasmic reticulum (ER). B. A part of the sporoblast showing the polar tube (PT) and the Golgi apparatus (G). C. Elongate sporoblast. Note the presence of the Golgi apparatus (G). D. Immature spore with 12 coils of polar tube. D: diplokaryon; ER: endoplasmic reticulum; N: nucleolus; P: polaroplast; PT: polar tube; PV: posterior vacuole. Scale Bars: A, B, C and D, 1 μm.
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Figure 3: Nosema podocotyloidis n. sp. A. Young sporoblast showing numerous electron-lucent vesicles (arrows and insert) and the endoplasmic reticulum (ER). B. A part of the sporoblast showing the polar tube (PT) and the Golgi apparatus (G). C. Elongate sporoblast. Note the presence of the Golgi apparatus (G). D. Immature spore with 12 coils of polar tube. D: diplokaryon; ER: endoplasmic reticulum; N: nucleolus; P: polaroplast; PT: polar tube; PV: posterior vacuole. Scale Bars: A, B, C and D, 1 μm.

Mentions: The young sporoblasts were ovoid cells with one central diplokaryon (Fig. 3A). They were bounded by an electron-dense coat with a thickness of approximately 30 nm. The electron-lucent vesicles of the cytoplasm increased in number and the endoplasmic reticulum became more distinct. The older sporoblasts were irregular or elongate (Fig. 3C) and their cytoplasm was full of ribosomes and lamellae of endoplasmic reticulum. The diplokaryon was less electron-dense than the cytoplasm. Distinct nucleoli were occasionally seen (Fig. 3C). The sporogenesis began with the development of a polar tube (Fig. 3B, C). Cross-sections of the immature polar tube appeared as symmetrical rings, each with an electron-dense central axis surrounded by a layer of electron-lucent material limited by a membrane (Fig. 3C). The diameter of the immature polar tube was approximately 80–120 nm. In the posterior end of some older sporoblasts, a prominent Golgi apparatus developed near the polar tube (Fig. 3B, C). Its structure was of traditional type. The polaroplast was the last spore structure formed and consisted of highly electron-dense bands (Fig. 3D). At this time, the formation of the endospore wall began. The young spores were diplokaryotic (Fig. 3D).Figure 3.


Ultrastructure and development of Nosema podocotyloidis n. sp. (Microsporidia), a hyperparasite of Podocotyloides magnatestis (Trematoda), a parasite of Parapristipoma octolineatum (Teleostei).

Toguebaye BS, Quilichini Y, Diagne PM, Marchand B - Parasite (2014)

Nosema podocotyloidis n. sp. A. Young sporoblast showing numerous electron-lucent vesicles (arrows and insert) and the endoplasmic reticulum (ER). B. A part of the sporoblast showing the polar tube (PT) and the Golgi apparatus (G). C. Elongate sporoblast. Note the presence of the Golgi apparatus (G). D. Immature spore with 12 coils of polar tube. D: diplokaryon; ER: endoplasmic reticulum; N: nucleolus; P: polaroplast; PT: polar tube; PV: posterior vacuole. Scale Bars: A, B, C and D, 1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150386&req=5

Figure 3: Nosema podocotyloidis n. sp. A. Young sporoblast showing numerous electron-lucent vesicles (arrows and insert) and the endoplasmic reticulum (ER). B. A part of the sporoblast showing the polar tube (PT) and the Golgi apparatus (G). C. Elongate sporoblast. Note the presence of the Golgi apparatus (G). D. Immature spore with 12 coils of polar tube. D: diplokaryon; ER: endoplasmic reticulum; N: nucleolus; P: polaroplast; PT: polar tube; PV: posterior vacuole. Scale Bars: A, B, C and D, 1 μm.
Mentions: The young sporoblasts were ovoid cells with one central diplokaryon (Fig. 3A). They were bounded by an electron-dense coat with a thickness of approximately 30 nm. The electron-lucent vesicles of the cytoplasm increased in number and the endoplasmic reticulum became more distinct. The older sporoblasts were irregular or elongate (Fig. 3C) and their cytoplasm was full of ribosomes and lamellae of endoplasmic reticulum. The diplokaryon was less electron-dense than the cytoplasm. Distinct nucleoli were occasionally seen (Fig. 3C). The sporogenesis began with the development of a polar tube (Fig. 3B, C). Cross-sections of the immature polar tube appeared as symmetrical rings, each with an electron-dense central axis surrounded by a layer of electron-lucent material limited by a membrane (Fig. 3C). The diameter of the immature polar tube was approximately 80–120 nm. In the posterior end of some older sporoblasts, a prominent Golgi apparatus developed near the polar tube (Fig. 3B, C). Its structure was of traditional type. The polaroplast was the last spore structure formed and consisted of highly electron-dense bands (Fig. 3D). At this time, the formation of the endospore wall began. The young spores were diplokaryotic (Fig. 3D).Figure 3.

Bottom Line: The polaroplast is divided into two regions: an outer electron-dense cup with granular content and lacking lamellae and an internal region, less electron-dense, composed of irregularly arranged sacs.The posterior vacuole, with an amorphous electron-dense content, is present.The new species is compared with other species of Nosema from trematodes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Parasitologie, Faculté des Sciences et Techniques, BP 5055, Dakar, République du Sénégal.

Show MeSH
Related in: MedlinePlus