Limits...
Emergence of human babesiosis along the border of China with Myanmar: detection by PCR and confirmation by sequencing.

Zhou X, Li SG, Wang JZ, Huang JL, Zhou HJ, Chen JH, Zhou XN - Emerg Microbes Infect (2014)

Bottom Line: The blood smears showed intraerythrocytic ring form and tetrads typical of small B. microti.In both cases, the rapid diagnostic test (RDT) ruled out the possibility of co-infections with malaria.Neither case was initially diagnosed because of the low Babesia parasitemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Medical College of Soochow University , Suzhou 215123, Jiangsu province, China ; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention , Shanghai 200025, China ; Key Laboratory of Parasite & Vector Biology, Ministry of Health, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis , Shanghai 200025, China.

ABSTRACT
Babesiosis is a tick-borne, zoonotic disease caused by Babesia spp. Two cases of babesiosis were detected by nested polymerase chain reaction (PCR) in Yunnan province, China, and further confirmed by molecular assay. The blood smears showed intraerythrocytic ring form and tetrads typical of small B. microti. In both cases, the rapid diagnostic test (RDT) ruled out the possibility of co-infections with malaria. Neither case was initially diagnosed because of the low Babesia parasitemia. These two cases of babesiosis in areas along the Myanmar-China border pose the question of the emergence of this under recognized infection in countries or areas where malaria is endemic.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees of the SSU rRNA and beta-tubulin gene sequences for the B. microti isolates obtained from the two patients in the China–Myanmar border area. Phylogenetic analysis produced by the neighbour-joining method using MEGA version 5.1 software. SSU rRNA and beta-tubulin gene sequences of our case study samples were denoted as Yunnan China in bold face. B. divergens and B. rodhaini were set as the outgroups. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150284&req=5

fig2: Phylogenetic trees of the SSU rRNA and beta-tubulin gene sequences for the B. microti isolates obtained from the two patients in the China–Myanmar border area. Phylogenetic analysis produced by the neighbour-joining method using MEGA version 5.1 software. SSU rRNA and beta-tubulin gene sequences of our case study samples were denoted as Yunnan China in bold face. B. divergens and B. rodhaini were set as the outgroups. Scale bar indicates nucleotide substitutions per site.

Mentions: Phylogenetic trees were produced by using the neighbor joining method in MEGA version 5.1 (http://mega.software.informer.com/5.1b/). The case samples (denoted as Yunnan China) clustered with other B. microti isolates. B. divergens and B. rodhaini were set as the outgroups (Figure 2).


Emergence of human babesiosis along the border of China with Myanmar: detection by PCR and confirmation by sequencing.

Zhou X, Li SG, Wang JZ, Huang JL, Zhou HJ, Chen JH, Zhou XN - Emerg Microbes Infect (2014)

Phylogenetic trees of the SSU rRNA and beta-tubulin gene sequences for the B. microti isolates obtained from the two patients in the China–Myanmar border area. Phylogenetic analysis produced by the neighbour-joining method using MEGA version 5.1 software. SSU rRNA and beta-tubulin gene sequences of our case study samples were denoted as Yunnan China in bold face. B. divergens and B. rodhaini were set as the outgroups. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150284&req=5

fig2: Phylogenetic trees of the SSU rRNA and beta-tubulin gene sequences for the B. microti isolates obtained from the two patients in the China–Myanmar border area. Phylogenetic analysis produced by the neighbour-joining method using MEGA version 5.1 software. SSU rRNA and beta-tubulin gene sequences of our case study samples were denoted as Yunnan China in bold face. B. divergens and B. rodhaini were set as the outgroups. Scale bar indicates nucleotide substitutions per site.
Mentions: Phylogenetic trees were produced by using the neighbor joining method in MEGA version 5.1 (http://mega.software.informer.com/5.1b/). The case samples (denoted as Yunnan China) clustered with other B. microti isolates. B. divergens and B. rodhaini were set as the outgroups (Figure 2).

Bottom Line: The blood smears showed intraerythrocytic ring form and tetrads typical of small B. microti.In both cases, the rapid diagnostic test (RDT) ruled out the possibility of co-infections with malaria.Neither case was initially diagnosed because of the low Babesia parasitemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Medical College of Soochow University , Suzhou 215123, Jiangsu province, China ; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention , Shanghai 200025, China ; Key Laboratory of Parasite & Vector Biology, Ministry of Health, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis , Shanghai 200025, China.

ABSTRACT
Babesiosis is a tick-borne, zoonotic disease caused by Babesia spp. Two cases of babesiosis were detected by nested polymerase chain reaction (PCR) in Yunnan province, China, and further confirmed by molecular assay. The blood smears showed intraerythrocytic ring form and tetrads typical of small B. microti. In both cases, the rapid diagnostic test (RDT) ruled out the possibility of co-infections with malaria. Neither case was initially diagnosed because of the low Babesia parasitemia. These two cases of babesiosis in areas along the Myanmar-China border pose the question of the emergence of this under recognized infection in countries or areas where malaria is endemic.

No MeSH data available.


Related in: MedlinePlus