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COUP-TFII regulates metastasis of colorectal adenocarcinoma cells by modulating Snail1.

Bao Y, Gu D, Feng W, Sun X, Wang X, Zhang X, Shi Q, Cui G, Yu H, Tang C, Deng A - Br. J. Cancer (2014)

Bottom Line: Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo.Chicken ovalbumin upstream promoter-transcription factor II regulated the transcription and expression of Snail1 by directly targeting the Snail1 promoter and regulated associated genes.Chicken ovalbumin upstream promoter-transcription factor II was found to be a biomarker associated with patient survival and colorectal cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital, Huzhou Teachers College, the First People's Hospital of Huzhou, Huzhou 313000, China.

ABSTRACT

Background: Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII, also known as NR2F2) promotes metastasis by functioning in the tumour microenvironment; however, the role of COUP-TFII in colorectal cancer remains unknown.

Methods: Human colon adenocarcinoma tissues were collected to test COUP-TFII expression. Wound-healing and cell invasion assay were used to evaluate migration and invasion of cells. Chicken ovalbumin upstream promoter-transcription factor II and related protein expression was assessed by immunostaining, immunoblotting and real-time PCR assay. Tamoxifen-inducible COUP-TFII knockout mice were employed to test COUP-TFII functions on colon cancer metastasis in vivo.

Results: Elevated expression of COUP-TFII in colorectal adenocarcinoma tissue correlated with overexpression of the Snail1 transcription factor. High COUP-TFII expression correlated with metastasis and shorter patient survival. Chicken ovalbumin upstream promoter-transcription factor II regulated the migration and invasion of cancer cells. With Snail1, COUP-TFII inhibited expression of adherence molecules such as ZO-1, E-cadherin and β-catenin in colorectal cancer cells. Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo. Chicken ovalbumin upstream promoter-transcription factor II regulated the transcription and expression of Snail1 by directly targeting the Snail1 promoter and regulated associated genes.

Conclusions: Chicken ovalbumin upstream promoter-transcription factor II was crucial for colorectal cancer metastasis and regulated cell migration and metastasis in conjunction with Snail1. Chicken ovalbumin upstream promoter-transcription factor II was found to be a biomarker associated with patient survival and colorectal cancer metastasis.

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Overexpression of COUP-TFII in intestinal epithelial cells results in tumour characteristics. (A) Expression of COUP-TFII and Snail-1 in HIEC, LOVO and HT29 cells by western blot. (B) HIEC cells were transfected with a COUP-TFII-overexpressing plasmid (COUP-TFII), and cytoplasmic COUP-TFII and Snail-1 were examined by western blot. (C) Wound-healing assay of control and COUP-TFII-overexpressing HIEC cells showing wound width for percent closure of the original wound in triplicate plates. Similar results were obtained in three experiments. (D) Migrated control and COUP-TFII-overexpressing cells that penetrated Matrigel-coated filters. (E) Snail-1 mRNA in HIEC, LOVO and HT29 cells was quantified by real-time PCR (*P< 0.01 compared to HIEC). (F) Snail-1 mRNA in control and COUP-TFII-overexpressing cells quantified by real-time PCR (*P<0.01 compared to CON).
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fig5: Overexpression of COUP-TFII in intestinal epithelial cells results in tumour characteristics. (A) Expression of COUP-TFII and Snail-1 in HIEC, LOVO and HT29 cells by western blot. (B) HIEC cells were transfected with a COUP-TFII-overexpressing plasmid (COUP-TFII), and cytoplasmic COUP-TFII and Snail-1 were examined by western blot. (C) Wound-healing assay of control and COUP-TFII-overexpressing HIEC cells showing wound width for percent closure of the original wound in triplicate plates. Similar results were obtained in three experiments. (D) Migrated control and COUP-TFII-overexpressing cells that penetrated Matrigel-coated filters. (E) Snail-1 mRNA in HIEC, LOVO and HT29 cells was quantified by real-time PCR (*P< 0.01 compared to HIEC). (F) Snail-1 mRNA in control and COUP-TFII-overexpressing cells quantified by real-time PCR (*P<0.01 compared to CON).

Mentions: Compared to cells from the normal intestinal epithelial line HIEC, colon cancer cells significantly overexpressed COUP-TFII (Figure 5A). To determine the effect of COUP-TFII on tumourigenesis of intestinal epithelial cells, we transformed HIEC cells with a COUP-TFII-overexpressing plasmid. The transformed cells showed high COUP-TFII and Snail1 expression (Figure 5B). E-cadherin expression was reduced, but vimentin expression that was identified as epithelial mesenchymal transition marker, was increased in COUP-TFII-overexpressing cells. Migration and invasion were also enhanced (Figure 5C and D) when cells were transfected with COUP-TFII plasmid. Real-time PCR showed that Snail1 mRNA was elevated in LOVO and HT29 cells and overexpression of COUP-TFII enhanced expression of Snail1 in HIEC cells (Figure 5E and F).


COUP-TFII regulates metastasis of colorectal adenocarcinoma cells by modulating Snail1.

Bao Y, Gu D, Feng W, Sun X, Wang X, Zhang X, Shi Q, Cui G, Yu H, Tang C, Deng A - Br. J. Cancer (2014)

Overexpression of COUP-TFII in intestinal epithelial cells results in tumour characteristics. (A) Expression of COUP-TFII and Snail-1 in HIEC, LOVO and HT29 cells by western blot. (B) HIEC cells were transfected with a COUP-TFII-overexpressing plasmid (COUP-TFII), and cytoplasmic COUP-TFII and Snail-1 were examined by western blot. (C) Wound-healing assay of control and COUP-TFII-overexpressing HIEC cells showing wound width for percent closure of the original wound in triplicate plates. Similar results were obtained in three experiments. (D) Migrated control and COUP-TFII-overexpressing cells that penetrated Matrigel-coated filters. (E) Snail-1 mRNA in HIEC, LOVO and HT29 cells was quantified by real-time PCR (*P< 0.01 compared to HIEC). (F) Snail-1 mRNA in control and COUP-TFII-overexpressing cells quantified by real-time PCR (*P<0.01 compared to CON).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150277&req=5

fig5: Overexpression of COUP-TFII in intestinal epithelial cells results in tumour characteristics. (A) Expression of COUP-TFII and Snail-1 in HIEC, LOVO and HT29 cells by western blot. (B) HIEC cells were transfected with a COUP-TFII-overexpressing plasmid (COUP-TFII), and cytoplasmic COUP-TFII and Snail-1 were examined by western blot. (C) Wound-healing assay of control and COUP-TFII-overexpressing HIEC cells showing wound width for percent closure of the original wound in triplicate plates. Similar results were obtained in three experiments. (D) Migrated control and COUP-TFII-overexpressing cells that penetrated Matrigel-coated filters. (E) Snail-1 mRNA in HIEC, LOVO and HT29 cells was quantified by real-time PCR (*P< 0.01 compared to HIEC). (F) Snail-1 mRNA in control and COUP-TFII-overexpressing cells quantified by real-time PCR (*P<0.01 compared to CON).
Mentions: Compared to cells from the normal intestinal epithelial line HIEC, colon cancer cells significantly overexpressed COUP-TFII (Figure 5A). To determine the effect of COUP-TFII on tumourigenesis of intestinal epithelial cells, we transformed HIEC cells with a COUP-TFII-overexpressing plasmid. The transformed cells showed high COUP-TFII and Snail1 expression (Figure 5B). E-cadherin expression was reduced, but vimentin expression that was identified as epithelial mesenchymal transition marker, was increased in COUP-TFII-overexpressing cells. Migration and invasion were also enhanced (Figure 5C and D) when cells were transfected with COUP-TFII plasmid. Real-time PCR showed that Snail1 mRNA was elevated in LOVO and HT29 cells and overexpression of COUP-TFII enhanced expression of Snail1 in HIEC cells (Figure 5E and F).

Bottom Line: Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo.Chicken ovalbumin upstream promoter-transcription factor II regulated the transcription and expression of Snail1 by directly targeting the Snail1 promoter and regulated associated genes.Chicken ovalbumin upstream promoter-transcription factor II was found to be a biomarker associated with patient survival and colorectal cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital, Huzhou Teachers College, the First People's Hospital of Huzhou, Huzhou 313000, China.

ABSTRACT

Background: Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII, also known as NR2F2) promotes metastasis by functioning in the tumour microenvironment; however, the role of COUP-TFII in colorectal cancer remains unknown.

Methods: Human colon adenocarcinoma tissues were collected to test COUP-TFII expression. Wound-healing and cell invasion assay were used to evaluate migration and invasion of cells. Chicken ovalbumin upstream promoter-transcription factor II and related protein expression was assessed by immunostaining, immunoblotting and real-time PCR assay. Tamoxifen-inducible COUP-TFII knockout mice were employed to test COUP-TFII functions on colon cancer metastasis in vivo.

Results: Elevated expression of COUP-TFII in colorectal adenocarcinoma tissue correlated with overexpression of the Snail1 transcription factor. High COUP-TFII expression correlated with metastasis and shorter patient survival. Chicken ovalbumin upstream promoter-transcription factor II regulated the migration and invasion of cancer cells. With Snail1, COUP-TFII inhibited expression of adherence molecules such as ZO-1, E-cadherin and β-catenin in colorectal cancer cells. Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo. Chicken ovalbumin upstream promoter-transcription factor II regulated the transcription and expression of Snail1 by directly targeting the Snail1 promoter and regulated associated genes.

Conclusions: Chicken ovalbumin upstream promoter-transcription factor II was crucial for colorectal cancer metastasis and regulated cell migration and metastasis in conjunction with Snail1. Chicken ovalbumin upstream promoter-transcription factor II was found to be a biomarker associated with patient survival and colorectal cancer metastasis.

Show MeSH
Related in: MedlinePlus