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Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.


Gross appearance of skin from control and rgVEGF164-treated sides of mice. After 3 weeks, rgVEGF164-treated animals displayed quick hair regrowth. Right side is rgVEGF164-treated side and left side is control. VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
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f4-ajas-27-9-1355: Gross appearance of skin from control and rgVEGF164-treated sides of mice. After 3 weeks, rgVEGF164-treated animals displayed quick hair regrowth. Right side is rgVEGF164-treated side and left side is control. VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.

Mentions: To determine the bioactivity of rgVEGF164 in promoting hair growth, mice were treated with rgVEGF164. Mice were shaved, and rgVEGF164 was applied topically on the shaved area, as described in Methods. The treatment was administered for 7 days in the first week, and after that animals were fed for 2 weeks without treatment. As a result, rgVEGF164-treated, but not control, animals experienced rapid hair regrowth in 3 weeks (Figure 4), indicating that rgVEGF164 promotes hair growth.


Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Gross appearance of skin from control and rgVEGF164-treated sides of mice. After 3 weeks, rgVEGF164-treated animals displayed quick hair regrowth. Right side is rgVEGF164-treated side and left side is control. VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150203&req=5

f4-ajas-27-9-1355: Gross appearance of skin from control and rgVEGF164-treated sides of mice. After 3 weeks, rgVEGF164-treated animals displayed quick hair regrowth. Right side is rgVEGF164-treated side and left side is control. VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
Mentions: To determine the bioactivity of rgVEGF164 in promoting hair growth, mice were treated with rgVEGF164. Mice were shaved, and rgVEGF164 was applied topically on the shaved area, as described in Methods. The treatment was administered for 7 days in the first week, and after that animals were fed for 2 weeks without treatment. As a result, rgVEGF164-treated, but not control, animals experienced rapid hair regrowth in 3 weeks (Figure 4), indicating that rgVEGF164 promotes hair growth.

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.