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Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.


Western blot analysis of rgVEGF164. (A) Western blot analysis of rgVEGF164 with monoclonal anti-His antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., Beijing, China). (B) Western blot analysis of rgVEGF164 with monoclonal anti-VEGF antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., China). VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
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f3-ajas-27-9-1355: Western blot analysis of rgVEGF164. (A) Western blot analysis of rgVEGF164 with monoclonal anti-His antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., Beijing, China). (B) Western blot analysis of rgVEGF164 with monoclonal anti-VEGF antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., China). VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.

Mentions: The rgVEGF164 was purified from the total protein lysate of pET-gVEGF164-transformed bacteria and electrophoresed. A single band of approximately 26.0 kDa appeared by 15% (w/v) SDS-PAGE (Figure 2B). After renaturation, the purified protein was identified by western blot using monoclonal anti-His (Figure 3A) and anti-VEGF antibodies (Figure 3B). Purified recombinant 6×his-gVEGF164 was then applied to the dorsal and lateral area of a shaved mouse.


Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Western blot analysis of rgVEGF164. (A) Western blot analysis of rgVEGF164 with monoclonal anti-His antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., Beijing, China). (B) Western blot analysis of rgVEGF164 with monoclonal anti-VEGF antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., China). VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4150203&req=5

f3-ajas-27-9-1355: Western blot analysis of rgVEGF164. (A) Western blot analysis of rgVEGF164 with monoclonal anti-His antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., Beijing, China). (B) Western blot analysis of rgVEGF164 with monoclonal anti-VEGF antibody. Lane 1, purified rgVEGF164; M, PM Western Midview Marker (CW0021, Beijing ComWin Biotech Co. Ltd., China). VEGF, vascular endothelial growth factor; rgVEGF164, recombinant goat VEFG164.
Mentions: The rgVEGF164 was purified from the total protein lysate of pET-gVEGF164-transformed bacteria and electrophoresed. A single band of approximately 26.0 kDa appeared by 15% (w/v) SDS-PAGE (Figure 2B). After renaturation, the purified protein was identified by western blot using monoclonal anti-His (Figure 3A) and anti-VEGF antibodies (Figure 3B). Purified recombinant 6×his-gVEGF164 was then applied to the dorsal and lateral area of a shaved mouse.

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.