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Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.


Schematic of insertion of goat VEGF164 cDNA into pET-his. VEGF, vascular endothelial growth factor.
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f1-ajas-27-9-1355: Schematic of insertion of goat VEGF164 cDNA into pET-his. VEGF, vascular endothelial growth factor.

Mentions: The pMD19-gVEGF164 was digested with BamH I and Hind III and subcloned into pET-his to obtain the pET-gVEGF164 expression vector (Figure 1). The pET-gVEGF164 was identified by restriction enzyme analysis, confirmed by sequencing, and transferred into E. coli BL21 (DE3) cells to express the 6×his-gVEGF164 recombinant protein.


Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Bao W, Yin J, Liang Y, Guo Z, Wang Y, Liu D, Wang X, Wang Z - Asian-australas. J. Anim. Sci. (2014)

Schematic of insertion of goat VEGF164 cDNA into pET-his. VEGF, vascular endothelial growth factor.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150203&req=5

f1-ajas-27-9-1355: Schematic of insertion of goat VEGF164 cDNA into pET-his. VEGF, vascular endothelial growth factor.
Mentions: The pMD19-gVEGF164 was digested with BamH I and Hind III and subcloned into pET-his to obtain the pET-gVEGF164 expression vector (Figure 1). The pET-gVEGF164 was identified by restriction enzyme analysis, confirmed by sequencing, and transferred into E. coli BL21 (DE3) cells to express the 6×his-gVEGF164 recombinant protein.

Bottom Line: The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C.The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group.Thus, rgVEGF164 increases hair growth in mice.

View Article: PubMed Central - PubMed

ABSTRACT
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

No MeSH data available.