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Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats.

Rahman MR, Rahman MM, Wan Khadijah WE, Abdullah RB - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively.In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages.The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; School of Agriculture and Rural Development, Bangladesh Open University, 1705 Gazipur, Bangladesh .

ABSTRACT
An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

No MeSH data available.


Correlation coefficient of body weight and estrus response for porcine follicle stimulating hormone-3.
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f3-ajas-27-9-1270: Correlation coefficient of body weight and estrus response for porcine follicle stimulating hormone-3.

Mentions: Although ovulation rate was similar for all the groups, pFSH-5 produced significantly a higher number of embryos than other groups. However, ovulation rate was not represented by embryos, which also included the ovulated oocytes that failed to be fertilized. It implies that the superovulation effect was not boosted up by high and low dosages of exogenous pFSH. In superovulated does, reduced fertility occurred due to prolonged elevation in estrogen concentrations resulting from the large anovulatory follicles and the abnormal maturation of oocytes (Cameron et al., 1988; Kumar et al., 1991; Ryan et al., 1991). Moreover, high estradiol concentration around and/or beyond ovulation induced disorders on the later reproductive process of fertilization and embryo development (Veiga-Lopez et al., 2006). Results of the present study also are in line with the findings of Ryan et al. (1991). In this study, the numbers of total and transferable embryo recovered from pFSH-3 and pFSH-8 were lower than pFSH-5. This result indicated that exogenous pFSH could promote ovarian responses, but the best superovulation results could be obtained only with a certain pFSH dosage range. However, the superovulation effect is not promoted by too high (>5 mg per kg BW) and too low (<5 mg per kg BW) dosages of exogenous pFSH as suggested in this study. Too high dosage of exogenous pFSH reduced the number for embryo production by producing a higher number of anovulatory follicles, which might increase estrogen levels in the blood. Elevated estrogen level in blood impaired sperm transportation in the female reproductive system and the breakage of sperm cells, which resulted in a higher number of unfertilized oocytes (Conley and Hawk, 1970; Armstrong et al., 1983; Greve et al., 1995). On the other hand, a too low dosage of exogenous pFSH may not be sufficient for stimulation of folliculogenesis and maturation of follicles. In this study, 50% of animal showed stimulation, which might be due to coincidence with the internal FSH production and the exogenous pFSH treatment. For pFSH-3, estrus response was not correlated with the BW (Figure 3).


Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats.

Rahman MR, Rahman MM, Wan Khadijah WE, Abdullah RB - Asian-australas. J. Anim. Sci. (2014)

Correlation coefficient of body weight and estrus response for porcine follicle stimulating hormone-3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150193&req=5

f3-ajas-27-9-1270: Correlation coefficient of body weight and estrus response for porcine follicle stimulating hormone-3.
Mentions: Although ovulation rate was similar for all the groups, pFSH-5 produced significantly a higher number of embryos than other groups. However, ovulation rate was not represented by embryos, which also included the ovulated oocytes that failed to be fertilized. It implies that the superovulation effect was not boosted up by high and low dosages of exogenous pFSH. In superovulated does, reduced fertility occurred due to prolonged elevation in estrogen concentrations resulting from the large anovulatory follicles and the abnormal maturation of oocytes (Cameron et al., 1988; Kumar et al., 1991; Ryan et al., 1991). Moreover, high estradiol concentration around and/or beyond ovulation induced disorders on the later reproductive process of fertilization and embryo development (Veiga-Lopez et al., 2006). Results of the present study also are in line with the findings of Ryan et al. (1991). In this study, the numbers of total and transferable embryo recovered from pFSH-3 and pFSH-8 were lower than pFSH-5. This result indicated that exogenous pFSH could promote ovarian responses, but the best superovulation results could be obtained only with a certain pFSH dosage range. However, the superovulation effect is not promoted by too high (>5 mg per kg BW) and too low (<5 mg per kg BW) dosages of exogenous pFSH as suggested in this study. Too high dosage of exogenous pFSH reduced the number for embryo production by producing a higher number of anovulatory follicles, which might increase estrogen levels in the blood. Elevated estrogen level in blood impaired sperm transportation in the female reproductive system and the breakage of sperm cells, which resulted in a higher number of unfertilized oocytes (Conley and Hawk, 1970; Armstrong et al., 1983; Greve et al., 1995). On the other hand, a too low dosage of exogenous pFSH may not be sufficient for stimulation of folliculogenesis and maturation of follicles. In this study, 50% of animal showed stimulation, which might be due to coincidence with the internal FSH production and the exogenous pFSH treatment. For pFSH-3, estrus response was not correlated with the BW (Figure 3).

Bottom Line: No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively.In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages.The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; School of Agriculture and Rural Development, Bangladesh Open University, 1705 Gazipur, Bangladesh .

ABSTRACT
An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

No MeSH data available.