Limits...
Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats.

Rahman MR, Rahman MM, Wan Khadijah WE, Abdullah RB - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively.In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages.The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; School of Agriculture and Rural Development, Bangladesh Open University, 1705 Gazipur, Bangladesh .

ABSTRACT
An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

No MeSH data available.


Recovered fresh embryos at 20× magnification.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150193&req=5

f1-ajas-27-9-1270: Recovered fresh embryos at 20× magnification.

Mentions: Embryo production performances as affected by different dosages of pFSH are presented in Table 2. The number of CL was increased from pFSH-3 to pFSH-5 and then decreased from pFSH-5 to pFSH-8, but CL percentages ([no. of CL/total no. stimulation]×100) showed an inverse relationship with the increase of pFSH dosage, and the values were 97.87%, 67.89%, and 48.76% for pFSH-3, pFSH-5, and pFSH-8, respectively. Although the average number of CL per doe for the three treated groups were similar, the total number of recovered embryos and transferable embryos per doe were significantly (p<0.05) higher in pFSH-5 (7.00 and 6.71) than in pFSH-3 (3.00 and 2.80) and in pFSH-8 (2.00 and 1.50), respectively (Figure 1). The number of structures (embryos plus unfertilized oocytes) recovered and the recovery rate per doe were higher in pFSH-5 (7.14 and 69.22) followed by pFSH-8 (4.25 and 60.74) and pFSH-3 (4.00 and 59.73), respectively (Table 2). The number of unfertilized oocytes per doe was lower in pFSH-5 (0.14) followed by pFSH-3 (1.00) and pFSH-8 (2.25), (Figure 2), but no differences (p>0.05) were observed among treatment groups.


Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats.

Rahman MR, Rahman MM, Wan Khadijah WE, Abdullah RB - Asian-australas. J. Anim. Sci. (2014)

Recovered fresh embryos at 20× magnification.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150193&req=5

f1-ajas-27-9-1270: Recovered fresh embryos at 20× magnification.
Mentions: Embryo production performances as affected by different dosages of pFSH are presented in Table 2. The number of CL was increased from pFSH-3 to pFSH-5 and then decreased from pFSH-5 to pFSH-8, but CL percentages ([no. of CL/total no. stimulation]×100) showed an inverse relationship with the increase of pFSH dosage, and the values were 97.87%, 67.89%, and 48.76% for pFSH-3, pFSH-5, and pFSH-8, respectively. Although the average number of CL per doe for the three treated groups were similar, the total number of recovered embryos and transferable embryos per doe were significantly (p<0.05) higher in pFSH-5 (7.00 and 6.71) than in pFSH-3 (3.00 and 2.80) and in pFSH-8 (2.00 and 1.50), respectively (Figure 1). The number of structures (embryos plus unfertilized oocytes) recovered and the recovery rate per doe were higher in pFSH-5 (7.14 and 69.22) followed by pFSH-8 (4.25 and 60.74) and pFSH-3 (4.00 and 59.73), respectively (Table 2). The number of unfertilized oocytes per doe was lower in pFSH-5 (0.14) followed by pFSH-3 (1.00) and pFSH-8 (2.25), (Figure 2), but no differences (p>0.05) were observed among treatment groups.

Bottom Line: No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively.In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages.The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; School of Agriculture and Rural Development, Bangladesh Open University, 1705 Gazipur, Bangladesh .

ABSTRACT
An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

No MeSH data available.