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Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.


Related in: MedlinePlus

Electrophoresis patterns of the DNA sequencing map and EcoRII and HinfI PCR-RFLP at porcine LEF-1 gene. (A) DNA sequencing map (upper) and electrophoresis pattern of the EcoR II PCR-RFLP (lower). The genotype “GG” demonstrated one undigested band (344 bp); Genotype “AA” showed two digested fragments (155 bp and 189 bp); Genotype “AG” showed three fragments (155, 189, and 344 bp). (B) DNA sequencing maps (upper) and electrophoresis pattern of the Hinf I PCR-RFLP (lower). The genotype “CC” demonstrated one undigested band (116 bp); genotype “TT” showed one digested fragment (89 bp) and; genotype “CT” showed two fragments (89 and 116 bp). Another digested fragment (27 bp) was invisible due to the lack of resolution within agarose gel. PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; LEF-1, lymphoid enhancer binding factor 1.
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f3-ajas-27-9-1254: Electrophoresis patterns of the DNA sequencing map and EcoRII and HinfI PCR-RFLP at porcine LEF-1 gene. (A) DNA sequencing map (upper) and electrophoresis pattern of the EcoR II PCR-RFLP (lower). The genotype “GG” demonstrated one undigested band (344 bp); Genotype “AA” showed two digested fragments (155 bp and 189 bp); Genotype “AG” showed three fragments (155, 189, and 344 bp). (B) DNA sequencing maps (upper) and electrophoresis pattern of the Hinf I PCR-RFLP (lower). The genotype “CC” demonstrated one undigested band (116 bp); genotype “TT” showed one digested fragment (89 bp) and; genotype “CT” showed two fragments (89 and 116 bp). Another digested fragment (27 bp) was invisible due to the lack of resolution within agarose gel. PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; LEF-1, lymphoid enhancer binding factor 1.

Mentions: The porcine LEF-1 gene is located in SSC8 and contains 153883 bases including 12 exons and 11 introns (Figure 2). The LEF-1 mRNA consists of 2357 nucleotides and codes 398 amino acids. Two novel mutations (NC_010450.3: g. 99514A>G, 119846C>T) were firstly detected by DNA pool sequencing in exon 4 and intron 9, respectively (Figure 3). No polymorphism was detected in the remaining sequences (P1–P3, P5–P9, P11, and P12) (Table 1) of the LEF-1 gene. The 99514A>G mutation resulted in a synonymous genetic code of proline, in detail, CCA (Pro)>CCG (Pro) at position 166 aa of LEF-1. The 119846C>T locus was observed in intron 9 and had no effect on amino acids changing.


Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

Electrophoresis patterns of the DNA sequencing map and EcoRII and HinfI PCR-RFLP at porcine LEF-1 gene. (A) DNA sequencing map (upper) and electrophoresis pattern of the EcoR II PCR-RFLP (lower). The genotype “GG” demonstrated one undigested band (344 bp); Genotype “AA” showed two digested fragments (155 bp and 189 bp); Genotype “AG” showed three fragments (155, 189, and 344 bp). (B) DNA sequencing maps (upper) and electrophoresis pattern of the Hinf I PCR-RFLP (lower). The genotype “CC” demonstrated one undigested band (116 bp); genotype “TT” showed one digested fragment (89 bp) and; genotype “CT” showed two fragments (89 and 116 bp). Another digested fragment (27 bp) was invisible due to the lack of resolution within agarose gel. PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; LEF-1, lymphoid enhancer binding factor 1.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4150191&req=5

f3-ajas-27-9-1254: Electrophoresis patterns of the DNA sequencing map and EcoRII and HinfI PCR-RFLP at porcine LEF-1 gene. (A) DNA sequencing map (upper) and electrophoresis pattern of the EcoR II PCR-RFLP (lower). The genotype “GG” demonstrated one undigested band (344 bp); Genotype “AA” showed two digested fragments (155 bp and 189 bp); Genotype “AG” showed three fragments (155, 189, and 344 bp). (B) DNA sequencing maps (upper) and electrophoresis pattern of the Hinf I PCR-RFLP (lower). The genotype “CC” demonstrated one undigested band (116 bp); genotype “TT” showed one digested fragment (89 bp) and; genotype “CT” showed two fragments (89 and 116 bp). Another digested fragment (27 bp) was invisible due to the lack of resolution within agarose gel. PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; LEF-1, lymphoid enhancer binding factor 1.
Mentions: The porcine LEF-1 gene is located in SSC8 and contains 153883 bases including 12 exons and 11 introns (Figure 2). The LEF-1 mRNA consists of 2357 nucleotides and codes 398 amino acids. Two novel mutations (NC_010450.3: g. 99514A>G, 119846C>T) were firstly detected by DNA pool sequencing in exon 4 and intron 9, respectively (Figure 3). No polymorphism was detected in the remaining sequences (P1–P3, P5–P9, P11, and P12) (Table 1) of the LEF-1 gene. The 99514A>G mutation resulted in a synonymous genetic code of proline, in detail, CCA (Pro)>CCG (Pro) at position 166 aa of LEF-1. The 119846C>T locus was observed in intron 9 and had no effect on amino acids changing.

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.


Related in: MedlinePlus