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Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.


The structure of porcine LEF-1 gene. Black boxes correspond to CDs. P1 to P12 denotes the fragments amplified by primers shown in Table 1. The SNP sites detected in present study were also included in this figure. LEF-1, lymphoid enhancer binding factor 1; CDs, coding sequence region; SNP, single nucleotide polymorphism.
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f2-ajas-27-9-1254: The structure of porcine LEF-1 gene. Black boxes correspond to CDs. P1 to P12 denotes the fragments amplified by primers shown in Table 1. The SNP sites detected in present study were also included in this figure. LEF-1, lymphoid enhancer binding factor 1; CDs, coding sequence region; SNP, single nucleotide polymorphism.

Mentions: The porcine LEF-1 gene is located in SSC8 and contains 153883 bases including 12 exons and 11 introns (Figure 2). The LEF-1 mRNA consists of 2357 nucleotides and codes 398 amino acids. Two novel mutations (NC_010450.3: g. 99514A>G, 119846C>T) were firstly detected by DNA pool sequencing in exon 4 and intron 9, respectively (Figure 3). No polymorphism was detected in the remaining sequences (P1–P3, P5–P9, P11, and P12) (Table 1) of the LEF-1 gene. The 99514A>G mutation resulted in a synonymous genetic code of proline, in detail, CCA (Pro)>CCG (Pro) at position 166 aa of LEF-1. The 119846C>T locus was observed in intron 9 and had no effect on amino acids changing.


Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

The structure of porcine LEF-1 gene. Black boxes correspond to CDs. P1 to P12 denotes the fragments amplified by primers shown in Table 1. The SNP sites detected in present study were also included in this figure. LEF-1, lymphoid enhancer binding factor 1; CDs, coding sequence region; SNP, single nucleotide polymorphism.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150191&req=5

f2-ajas-27-9-1254: The structure of porcine LEF-1 gene. Black boxes correspond to CDs. P1 to P12 denotes the fragments amplified by primers shown in Table 1. The SNP sites detected in present study were also included in this figure. LEF-1, lymphoid enhancer binding factor 1; CDs, coding sequence region; SNP, single nucleotide polymorphism.
Mentions: The porcine LEF-1 gene is located in SSC8 and contains 153883 bases including 12 exons and 11 introns (Figure 2). The LEF-1 mRNA consists of 2357 nucleotides and codes 398 amino acids. Two novel mutations (NC_010450.3: g. 99514A>G, 119846C>T) were firstly detected by DNA pool sequencing in exon 4 and intron 9, respectively (Figure 3). No polymorphism was detected in the remaining sequences (P1–P3, P5–P9, P11, and P12) (Table 1) of the LEF-1 gene. The 99514A>G mutation resulted in a synonymous genetic code of proline, in detail, CCA (Pro)>CCG (Pro) at position 166 aa of LEF-1. The 119846C>T locus was observed in intron 9 and had no effect on amino acids changing.

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.