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Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.


Related in: MedlinePlus

LEF-1 mRNA levels in Guanzhong Black pigs. (A) LEF-1 mRNA levels in 8 tissues of 3-day-old Guanzhong Black pigs, including teat, heart, liver, subcutaneous fat, spleen, kidney, lung and muscle tissues were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (B) LEF-1 mRNA levels in 7 tissues of 180-day-old Guanzhong Black pigs, including teat, heart, liver, spleen, lymph, thyroid and fat tissues, were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (C) Comparative analysis of LEF-1 mRNA expression in Guanzhong Black pigs teat tissues during 3 growth stages. All the mRNA levels were normalized to LEF-1 mRNA in 180-day-old. LEF-1, lymphoid enhancer binding factor 1.
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f1-ajas-27-9-1254: LEF-1 mRNA levels in Guanzhong Black pigs. (A) LEF-1 mRNA levels in 8 tissues of 3-day-old Guanzhong Black pigs, including teat, heart, liver, subcutaneous fat, spleen, kidney, lung and muscle tissues were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (B) LEF-1 mRNA levels in 7 tissues of 180-day-old Guanzhong Black pigs, including teat, heart, liver, spleen, lymph, thyroid and fat tissues, were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (C) Comparative analysis of LEF-1 mRNA expression in Guanzhong Black pigs teat tissues during 3 growth stages. All the mRNA levels were normalized to LEF-1 mRNA in 180-day-old. LEF-1, lymphoid enhancer binding factor 1.

Mentions: In 3-day-old Guanzhong Black pigs, we detected the relative mRNA levels of porcine LEF-1 in teat, heart, liver, subcutaneous fat, spleen, kidney, lung and longissimus muscle tissues (Figure 1A). The data showed a wide range of expression of LEF-1 in different porcine tissues. In detail, relatively high levels in teat, spleen, kidney and lung tissue (about 25.6, 7.3, 10.3, and 11.9 fold to heart tissue respectively) were observed As well as low levels in subcutaneous fat tissue and longissimus muscle tissue.


Association of Novel Polymorphisms in Lymphoid Enhancer Binding Factor 1 (LEF-1) Gene with Number of Teats in Different Breeds of Pig.

Xu RX, Wei N, Wang Y, Wang GQ, Yang GS, Pang WJ - Asian-australas. J. Anim. Sci. (2014)

LEF-1 mRNA levels in Guanzhong Black pigs. (A) LEF-1 mRNA levels in 8 tissues of 3-day-old Guanzhong Black pigs, including teat, heart, liver, subcutaneous fat, spleen, kidney, lung and muscle tissues were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (B) LEF-1 mRNA levels in 7 tissues of 180-day-old Guanzhong Black pigs, including teat, heart, liver, spleen, lymph, thyroid and fat tissues, were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (C) Comparative analysis of LEF-1 mRNA expression in Guanzhong Black pigs teat tissues during 3 growth stages. All the mRNA levels were normalized to LEF-1 mRNA in 180-day-old. LEF-1, lymphoid enhancer binding factor 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150191&req=5

f1-ajas-27-9-1254: LEF-1 mRNA levels in Guanzhong Black pigs. (A) LEF-1 mRNA levels in 8 tissues of 3-day-old Guanzhong Black pigs, including teat, heart, liver, subcutaneous fat, spleen, kidney, lung and muscle tissues were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (B) LEF-1 mRNA levels in 7 tissues of 180-day-old Guanzhong Black pigs, including teat, heart, liver, spleen, lymph, thyroid and fat tissues, were detected. All the mRNA levels were normalized to LEF-1 mRNA of heart; (C) Comparative analysis of LEF-1 mRNA expression in Guanzhong Black pigs teat tissues during 3 growth stages. All the mRNA levels were normalized to LEF-1 mRNA in 180-day-old. LEF-1, lymphoid enhancer binding factor 1.
Mentions: In 3-day-old Guanzhong Black pigs, we detected the relative mRNA levels of porcine LEF-1 in teat, heart, liver, subcutaneous fat, spleen, kidney, lung and longissimus muscle tissues (Figure 1A). The data showed a wide range of expression of LEF-1 in different porcine tissues. In detail, relatively high levels in teat, spleen, kidney and lung tissue (about 25.6, 7.3, 10.3, and 11.9 fold to heart tissue respectively) were observed As well as low levels in subcutaneous fat tissue and longissimus muscle tissue.

Bottom Line: Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait.Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds.The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT".

View Article: PubMed Central - PubMed

ABSTRACT
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

No MeSH data available.


Related in: MedlinePlus