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Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization.

Lee WY, Lee R, Kim HC, Lee KH, Cui XS, Kim NH, Kim SH, Lee IJ, Uhm SJ, Yoon MJ, Song H - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology.The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances.In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, College of Agriculture, Chungbuk National University, Choung-ju 361-763, Korea .

ABSTRACT
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

No MeSH data available.


Comparison of pronucleus formation after in vitro fertilization of porcine oocytes. (A) Porcine oocytes in vitro fertilized with sperm from boar number 2012 and (B) 2023. White arrows indicate pronuclei, the red arrow indicates a polar body of unfertilized oocytes, and yellow circles indicate non-fertilized sperm heads containing nucleic acid (4′,6-diamidino-2-phenylindole, ×100).
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f4-ajas-27-10-1417: Comparison of pronucleus formation after in vitro fertilization of porcine oocytes. (A) Porcine oocytes in vitro fertilized with sperm from boar number 2012 and (B) 2023. White arrows indicate pronuclei, the red arrow indicates a polar body of unfertilized oocytes, and yellow circles indicate non-fertilized sperm heads containing nucleic acid (4′,6-diamidino-2-phenylindole, ×100).

Mentions: To compare and identify the fertilization ability of sperm from boar 2012 and 2023, IVF was performed using in vitro matured pig oocytes. Sperm from boar 2012 and 2023 showed 3.4 and 75.45% of fertilization rate, respectively (Table 2). Interestingly, the number of oocytes presenting a pronucleus (PN) formation using sperm from boar 2012 was only four, one oocyte had 1PN and 3 oocytes had 2PN. However, sperm from boar 2023 fertilized 83 eggs out of 110 oocytes, and the number of PN in fertilized eggs varied from one to more than three (Table 2). Most of the spermatozoa from boar 2012 did not penetrate the ZP of oocytes and failed to form PN. They remained on the outer area of the oocytes (Figure 4A). In contrast, a lower number of sperm nuclei were identified on the ZP region in fertilized oocytes with sperm from boar 2023 and PN formation was determined (Figure 4B).


Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization.

Lee WY, Lee R, Kim HC, Lee KH, Cui XS, Kim NH, Kim SH, Lee IJ, Uhm SJ, Yoon MJ, Song H - Asian-australas. J. Anim. Sci. (2014)

Comparison of pronucleus formation after in vitro fertilization of porcine oocytes. (A) Porcine oocytes in vitro fertilized with sperm from boar number 2012 and (B) 2023. White arrows indicate pronuclei, the red arrow indicates a polar body of unfertilized oocytes, and yellow circles indicate non-fertilized sperm heads containing nucleic acid (4′,6-diamidino-2-phenylindole, ×100).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150174&req=5

f4-ajas-27-10-1417: Comparison of pronucleus formation after in vitro fertilization of porcine oocytes. (A) Porcine oocytes in vitro fertilized with sperm from boar number 2012 and (B) 2023. White arrows indicate pronuclei, the red arrow indicates a polar body of unfertilized oocytes, and yellow circles indicate non-fertilized sperm heads containing nucleic acid (4′,6-diamidino-2-phenylindole, ×100).
Mentions: To compare and identify the fertilization ability of sperm from boar 2012 and 2023, IVF was performed using in vitro matured pig oocytes. Sperm from boar 2012 and 2023 showed 3.4 and 75.45% of fertilization rate, respectively (Table 2). Interestingly, the number of oocytes presenting a pronucleus (PN) formation using sperm from boar 2012 was only four, one oocyte had 1PN and 3 oocytes had 2PN. However, sperm from boar 2023 fertilized 83 eggs out of 110 oocytes, and the number of PN in fertilized eggs varied from one to more than three (Table 2). Most of the spermatozoa from boar 2012 did not penetrate the ZP of oocytes and failed to form PN. They remained on the outer area of the oocytes (Figure 4A). In contrast, a lower number of sperm nuclei were identified on the ZP region in fertilized oocytes with sperm from boar 2023 and PN formation was determined (Figure 4B).

Bottom Line: Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology.The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances.In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, College of Agriculture, Chungbuk National University, Choung-ju 361-763, Korea .

ABSTRACT
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

No MeSH data available.